Perforin-2 is essential for intracellular defense of parenchymal cells and phagocytes against pathogenic bacteria

Ryan M. McCormack, Lesley R. de Armas, Motoaki Shiratsuchi, Desiree G. Fiorentino, Melissa L. Olsson, Mathias G. Lichtenheld, Alejo Morales, Kirill Lyapichev, Louis E. Gonzalez, Natasa Strbo, Neelima Sukumar, Olivera Stojadinovic, Gregory V. Plano, George P. Munson, Marjana Tomic-Canic, Robert S. Kirsner, David G. Russell, Eckhard R. Podack

研究成果: ジャーナルへの寄稿記事

17 引用 (Scopus)

抄録

Perforin-2 (MPEG1) is a pore-forming, antibacterial protein with broad-spectrum activity. Perforin-2 is expressed constitutively in phagocytes and inducibly in parenchymal, tissue-forming cells. In vitro, Perforin-2 prevents the intracellular replication and proliferation of bacterial pathogens in these cells. Perforin-2 knockout mice are unable to control the systemic dissemination of methicillin-resistant Staphylococcus aureus (MRSA) or Salmonella typhimurium and perish shortly after epicutaneous or orogastric infection respectively. In contrast, Perforin-2-sufficient littermates clear the infection. Perforin-2 is a transmembrane protein of cytosolic vesicles -derived from multiple organelles- that translocate to and fuse with bacterium containing vesicles. Subsequently, Perforin-2 polymerizes and forms large clusters of 100 Å pores in the bacterial surface with Perforin-2 cleavage products present in bacteria. Perforin-2 is also required for the bactericidal activity of reactive oxygen and nitrogen species and hydrolytic enzymes. Perforin-2 constitutes a novel and apparently essential bactericidal effector molecule of the innate immune system.

元の言語英語
記事番号e06508
ジャーナルeLife
4
発行部数September2015
DOI
出版物ステータス出版済み - 9 24 2015
外部発表Yes

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Phagocytes
Bacteria
perforin 2
Reactive Nitrogen Species
Salmonella
Methicillin
Immune system
Pathogens
Electric fuses
Salmonella typhimurium
Methicillin-Resistant Staphylococcus aureus
Infection
Knockout Mice
Organelles
Immune System
Reactive Oxygen Species
Proteins
Tissue
Molecules
Enzymes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

これを引用

McCormack, R. M., de Armas, L. R., Shiratsuchi, M., Fiorentino, D. G., Olsson, M. L., Lichtenheld, M. G., ... Podack, E. R. (2015). Perforin-2 is essential for intracellular defense of parenchymal cells and phagocytes against pathogenic bacteria. eLife, 4(September2015), [e06508]. https://doi.org/10.7554/eLife.06508

Perforin-2 is essential for intracellular defense of parenchymal cells and phagocytes against pathogenic bacteria. / McCormack, Ryan M.; de Armas, Lesley R.; Shiratsuchi, Motoaki; Fiorentino, Desiree G.; Olsson, Melissa L.; Lichtenheld, Mathias G.; Morales, Alejo; Lyapichev, Kirill; Gonzalez, Louis E.; Strbo, Natasa; Sukumar, Neelima; Stojadinovic, Olivera; Plano, Gregory V.; Munson, George P.; Tomic-Canic, Marjana; Kirsner, Robert S.; Russell, David G.; Podack, Eckhard R.

:: eLife, 巻 4, 番号 September2015, e06508, 24.09.2015.

研究成果: ジャーナルへの寄稿記事

McCormack, RM, de Armas, LR, Shiratsuchi, M, Fiorentino, DG, Olsson, ML, Lichtenheld, MG, Morales, A, Lyapichev, K, Gonzalez, LE, Strbo, N, Sukumar, N, Stojadinovic, O, Plano, GV, Munson, GP, Tomic-Canic, M, Kirsner, RS, Russell, DG & Podack, ER 2015, 'Perforin-2 is essential for intracellular defense of parenchymal cells and phagocytes against pathogenic bacteria', eLife, 巻. 4, 番号 September2015, e06508. https://doi.org/10.7554/eLife.06508
McCormack RM, de Armas LR, Shiratsuchi M, Fiorentino DG, Olsson ML, Lichtenheld MG その他. Perforin-2 is essential for intracellular defense of parenchymal cells and phagocytes against pathogenic bacteria. eLife. 2015 9 24;4(September2015). e06508. https://doi.org/10.7554/eLife.06508
McCormack, Ryan M. ; de Armas, Lesley R. ; Shiratsuchi, Motoaki ; Fiorentino, Desiree G. ; Olsson, Melissa L. ; Lichtenheld, Mathias G. ; Morales, Alejo ; Lyapichev, Kirill ; Gonzalez, Louis E. ; Strbo, Natasa ; Sukumar, Neelima ; Stojadinovic, Olivera ; Plano, Gregory V. ; Munson, George P. ; Tomic-Canic, Marjana ; Kirsner, Robert S. ; Russell, David G. ; Podack, Eckhard R. / Perforin-2 is essential for intracellular defense of parenchymal cells and phagocytes against pathogenic bacteria. :: eLife. 2015 ; 巻 4, 番号 September2015.
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abstract = "Perforin-2 (MPEG1) is a pore-forming, antibacterial protein with broad-spectrum activity. Perforin-2 is expressed constitutively in phagocytes and inducibly in parenchymal, tissue-forming cells. In vitro, Perforin-2 prevents the intracellular replication and proliferation of bacterial pathogens in these cells. Perforin-2 knockout mice are unable to control the systemic dissemination of methicillin-resistant Staphylococcus aureus (MRSA) or Salmonella typhimurium and perish shortly after epicutaneous or orogastric infection respectively. In contrast, Perforin-2-sufficient littermates clear the infection. Perforin-2 is a transmembrane protein of cytosolic vesicles -derived from multiple organelles- that translocate to and fuse with bacterium containing vesicles. Subsequently, Perforin-2 polymerizes and forms large clusters of 100 {\AA} pores in the bacterial surface with Perforin-2 cleavage products present in bacteria. Perforin-2 is also required for the bactericidal activity of reactive oxygen and nitrogen species and hydrolytic enzymes. Perforin-2 constitutes a novel and apparently essential bactericidal effector molecule of the innate immune system.",
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AU - McCormack, Ryan M.

AU - de Armas, Lesley R.

AU - Shiratsuchi, Motoaki

AU - Fiorentino, Desiree G.

AU - Olsson, Melissa L.

AU - Lichtenheld, Mathias G.

AU - Morales, Alejo

AU - Lyapichev, Kirill

AU - Gonzalez, Louis E.

AU - Strbo, Natasa

AU - Sukumar, Neelima

AU - Stojadinovic, Olivera

AU - Plano, Gregory V.

AU - Munson, George P.

AU - Tomic-Canic, Marjana

AU - Kirsner, Robert S.

AU - Russell, David G.

AU - Podack, Eckhard R.

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