Periploca sepium Bunge as a model plant for rubber biosynthesis study

Takeshi Bamba, Tomoki Sando, Asuka Miyabashira, Koichiro Gyokusen, Yoshihisa Nakazawa, Yinquan Su, Eiichiro Fukusaki, Akio Kobayashi

研究成果: ジャーナルへの寄稿記事

8 引用 (Scopus)

抄録

Periploca sepium Bunge (Chinese silk vine) is a woody climbing vine belonging to the family Asclepiadaceae. It originally comes from Northwest China. Periploca resembles the Para-rubber tree, Hevea brasiliensis, regarding a similar body plan to produce a milky exudate containing rubber latex. The Periploca plant was assessed as a rubber-producing plant by rubber structure elucidation and its molecular weight distribution. A rubber fraction purified from the milky exudate was subjected to 1H NMR analysis, and a characteristic signal derived from cis-polyisoprene was observed. In addition, when the molecular weight distribution of rubber components in the exudate was measured (using size-exclusion chromatography), the number-average molecular weight (Mn), weight-average molecular weight (Mw), and polydispersity (Mw/Mn) were estimated to be Mn = 1.3 × 105, Mw = 4.1 × 10 5, and Mw/Mn = 3.1, respectively. Furthermore, the presence of polyisoprene, with Mn = 4.0 × 104, Mw = 7.6 × 10 4, and Mw/Mn = 2.5, was also confirmed in plantlets obtained from shoots as a result of tissue culture.

元の言語英語
ページ(範囲)579-582
ページ数4
ジャーナルZeitschrift fur Naturforschung - Section C Journal of Biosciences
62
発行部数7-8
DOI
出版物ステータス出版済み - 1 1 2007

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Periploca
Sepia
Biosynthesis
Rubber
Molecular Weight
Molecular weight
Exudates and Transudates
Polyisoprenes
Hevea
Molecular weight distribution
Tissue culture
Silk
Size exclusion chromatography
Apocynaceae
Polydispersity
Plant Structures
Nuclear magnetic resonance
Gel Chromatography
China

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

これを引用

Periploca sepium Bunge as a model plant for rubber biosynthesis study. / Bamba, Takeshi; Sando, Tomoki; Miyabashira, Asuka; Gyokusen, Koichiro; Nakazawa, Yoshihisa; Su, Yinquan; Fukusaki, Eiichiro; Kobayashi, Akio.

:: Zeitschrift fur Naturforschung - Section C Journal of Biosciences, 巻 62, 番号 7-8, 01.01.2007, p. 579-582.

研究成果: ジャーナルへの寄稿記事

Bamba, T, Sando, T, Miyabashira, A, Gyokusen, K, Nakazawa, Y, Su, Y, Fukusaki, E & Kobayashi, A 2007, 'Periploca sepium Bunge as a model plant for rubber biosynthesis study', Zeitschrift fur Naturforschung - Section C Journal of Biosciences, 巻. 62, 番号 7-8, pp. 579-582. https://doi.org/10.1515/znc-2007-7-820
Bamba, Takeshi ; Sando, Tomoki ; Miyabashira, Asuka ; Gyokusen, Koichiro ; Nakazawa, Yoshihisa ; Su, Yinquan ; Fukusaki, Eiichiro ; Kobayashi, Akio. / Periploca sepium Bunge as a model plant for rubber biosynthesis study. :: Zeitschrift fur Naturforschung - Section C Journal of Biosciences. 2007 ; 巻 62, 番号 7-8. pp. 579-582.
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