We found that γδ T cells increased in number in the peritoneal cavity after i.p. inoculation with Escherichia coli (ATCC 26) in mice. The increase of the γδ T cells was most prominent on day 5 after inoculation when the pathogens had been already eliminated from the hosts. Two-color flow cytometric (FCM) analysis revealed that these γδ T cells in infected C57BL/6 mice expressed Thy-1 Ag on their cell surface. On the other hand, γδ T cells induced by E. coli inoculation in C3H/He mice contained Thy-1- negative γδ T cells in addition to the Thy-1-positive γδ T cells. In both strains, irrespective of Thy-1 Ag expression, these γδ T cells were CD5 negative, CD44 positive, L-selectin negative, Ly-6C negative, and IL-2R low positive. Analyses of peritoneal exudate cells (PEC) from several other strains of mice after E. coli inoculation suggested that Thy-1-negative γδ T cells appear in mice carrying endogenous superantigen specific for Vβ3, especially mammary tumor virus-6. These findings suggest that Thy-1 Ag expression on the γδ T cells appearing in the peritoneal cavity after i.p. E. coli inoculation is correlated to the Mls phenotype of the host mice.
|ジャーナル||Journal of Immunology|
|出版物ステータス||出版済み - 1993|
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