Periventricular notch activation and asymmetric Ngn2 and Tbr2 expression in pair-generated neocortical daughter cells

Wataru Ochiai, Sayaka Nakatani, Taishi Takahara, Masahiko Kainuma, Makoto Masaoka, Sayaka Minobe, Masakazu Namihira, Kinichi Nakashima, Akira Sakakibara, Masaharu Ogawa, Takaki Miyata

研究成果: Contribution to journalArticle査読

80 被引用数 (Scopus)

抄録

To understand the cellular and molecular mechanisms regulating cytogenesis within the neocortical ventricular zone, we examined at high resolution the spatiotemporal expression patterns of Ngn2 and Tbr2. Individually DiI-labeled daughter cells were tracked from their birth in slice cultures and immunostained for Ngn2 and Tbr2. Both proteins were initially absent from daughter cells during the first 2 h. Ngn2 expression was then initiated asymmetrically in one of the daughter cells, with a bias towards the apical cell, followed by a similar pattern of expression for Tbr2, which we found to be a direct target of Ngn2. How this asymmetric Ngn2 expression is achieved is unclear, but γ-secretase inhibition at the birth of daughter cells resulted in premature Ngn2 expression, suggesting that Notch signaling in nascent daughter cells suppresses a Ngn2-Tbr2 cascade. Many of the nascent cells exhibited pin-like morphology with a short ventricular process, suggesting periventricular presentation of Notch ligands to these cells.

本文言語英語
ページ(範囲)225-233
ページ数9
ジャーナルMolecular and Cellular Neuroscience
40
2
DOI
出版ステータス出版済み - 2 2009
外部発表はい

All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 細胞および分子神経科学
  • 細胞生物学

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