TY - JOUR
T1 - Persistence of hepatitis C virus RNA in established human hepatocellular carcinoma cell lines
AU - Tsuboi, So
AU - Nagamori, Seishi
AU - Miyazaki, Masahiro
AU - Mihara, Koichiro
AU - Fukaya, Kenichi
AU - Teruya, Kiichiro
AU - Kosaka, Tsunenori
AU - Tsuji, Takao
AU - Namba, Masayoshi
N1 - Copyright:
Copyright 2007 Elsevier B.V., All rights reserved.
PY - 1996/2
Y1 - 1996/2
N2 - The persistence of the viral RNA of hepatitis C virus (HCV) was examined in 13 hepatocellular carcinoma (HCC) and two hepatoblastoma cell lines by reverse transcription followed by the polymerase chain reaction (RT-PCR). HCV RNA was detected in three HCC lines (JHH-1, JHH-4, and JHH-6) and negative-strand viral RNA was found in JHH-4, indicating that there is a putative replicative intermediate of HCV in JHH-4 cells. To rule out the possibility of contamination, the partial nucleotide sequences of HCV-specific PCR products of these three cell lines were determined. The clone from JHH-1 belonged to genotype 1 (1 a or 1b), and the clones from JHH-4 and JHH-6 belonged to genotype 2b, but their sequences differed from each other. These cell lines may be useful for studies related to HCV.
AB - The persistence of the viral RNA of hepatitis C virus (HCV) was examined in 13 hepatocellular carcinoma (HCC) and two hepatoblastoma cell lines by reverse transcription followed by the polymerase chain reaction (RT-PCR). HCV RNA was detected in three HCC lines (JHH-1, JHH-4, and JHH-6) and negative-strand viral RNA was found in JHH-4, indicating that there is a putative replicative intermediate of HCV in JHH-4 cells. To rule out the possibility of contamination, the partial nucleotide sequences of HCV-specific PCR products of these three cell lines were determined. The clone from JHH-1 belonged to genotype 1 (1 a or 1b), and the clones from JHH-4 and JHH-6 belonged to genotype 2b, but their sequences differed from each other. These cell lines may be useful for studies related to HCV.
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U2 - 10.1002/(SICI)1096-9071(199602)48:2<133::AID-JMV3>3.0.CO;2-A
DO - 10.1002/(SICI)1096-9071(199602)48:2<133::AID-JMV3>3.0.CO;2-A
M3 - Article
C2 - 8835345
AN - SCOPUS:0030041322
VL - 48
SP - 133
EP - 140
JO - Journal of Medical Virology
JF - Journal of Medical Virology
SN - 0146-6615
IS - 2
ER -