Pex5p stabilizes Pex14p: A study using a newly isolated pex5 CHO cell mutant, ZPEG101

Ryuichi Natsuyama, Kanji Okumoto, Yukio Fujiki

研究成果: ジャーナルへの寄稿記事

10 引用 (Scopus)

抄録

Pex5p [PTS (peroxisome-targeting signal) type 1 receptor] plays an essential role in peroxisomal matrix protein import. In the present study, we isolated a novel PEX5-deficient CHO (Chinese-hamster ovary) cell mutant, termed ZPEG101, showing typical peroxisomal import defects of both PTS1 and PTS2 proteins. ZPEG101 is distinct from other known pex5 CHO mutants in its Pex5p expression. An undetectable level of Pex5p in ZPEG101 results in unstable Pex14p, which is due to inefficient translocation to the peroxisomal membrane. All of the mutant phenotypes of ZPEG101 are restored by expression of wild-type Pex5pL, a longer form of Pex5p, suggesting a role for Pex5p in sustaining the levels of Pex14p in addition to peroxisomalmatrix protein import. Complementation analysis using various Pex5p mutants revealed that in the seven pentapeptide WXXXF/Y motifs in Pex5pL, known as the multiple binding sites for Pex14p, the fifth motif is an auxiliary binding site for Pex14p and is required for Pex14p stability. Furthermore, we found that Pex5p-Pex13p interaction is essential for the import of PTS1 proteins as well as catalase, but not for that of PTS2 proteins. Therefore ZPEG101 with no Pex5p would be a useful tool for investigating Pex5p function and delineating the mechanisms underlying peroxisomal matrix protein import.

元の言語英語
ページ(範囲)195-207
ページ数13
ジャーナルBiochemical Journal
449
発行部数1
DOI
出版物ステータス出版済み - 1 1 2013

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Cricetulus
Ovary
Proteins
Binding Sites
Catalase
Cells
Membranes
Phenotype
Defects
peroxisomal targeting signal 2 receptor

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

Pex5p stabilizes Pex14p : A study using a newly isolated pex5 CHO cell mutant, ZPEG101. / Natsuyama, Ryuichi; Okumoto, Kanji; Fujiki, Yukio.

:: Biochemical Journal, 巻 449, 番号 1, 01.01.2013, p. 195-207.

研究成果: ジャーナルへの寄稿記事

Natsuyama, Ryuichi ; Okumoto, Kanji ; Fujiki, Yukio. / Pex5p stabilizes Pex14p : A study using a newly isolated pex5 CHO cell mutant, ZPEG101. :: Biochemical Journal. 2013 ; 巻 449, 番号 1. pp. 195-207.
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abstract = "Pex5p [PTS (peroxisome-targeting signal) type 1 receptor] plays an essential role in peroxisomal matrix protein import. In the present study, we isolated a novel PEX5-deficient CHO (Chinese-hamster ovary) cell mutant, termed ZPEG101, showing typical peroxisomal import defects of both PTS1 and PTS2 proteins. ZPEG101 is distinct from other known pex5 CHO mutants in its Pex5p expression. An undetectable level of Pex5p in ZPEG101 results in unstable Pex14p, which is due to inefficient translocation to the peroxisomal membrane. All of the mutant phenotypes of ZPEG101 are restored by expression of wild-type Pex5pL, a longer form of Pex5p, suggesting a role for Pex5p in sustaining the levels of Pex14p in addition to peroxisomalmatrix protein import. Complementation analysis using various Pex5p mutants revealed that in the seven pentapeptide WXXXF/Y motifs in Pex5pL, known as the multiple binding sites for Pex14p, the fifth motif is an auxiliary binding site for Pex14p and is required for Pex14p stability. Furthermore, we found that Pex5p-Pex13p interaction is essential for the import of PTS1 proteins as well as catalase, but not for that of PTS2 proteins. Therefore ZPEG101 with no Pex5p would be a useful tool for investigating Pex5p function and delineating the mechanisms underlying peroxisomal matrix protein import.",
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