Pharmacologic unmasking of epigenetically silenced tumor suppressor genes in esophageal squamous cell carcinoma

Keishi Yamashita, Sunil Upadhyay, Motonobu Osada, Mohammad O. Hoque, Yan Xiao, Masaki Mori, Fumiaki Sato, Stephen J. Meltzer, David Sidransky

研究成果: ジャーナルへの寄稿学術誌査読

287 被引用数 (Scopus)

抄録

We performed a comprehensive survey of commonly inactivated tumor suppressor genes in esophageal squamous cell carcinoma (ESCC) based on functional reactivation of epigenetically silenced tumor suppressor genes by 5-aza-2′-deoxycytidine and trichostatin A using microarrays containing 12599 genes. Among 58 genes identified by this approach, 44 (76%) harbored dense CpG islands in the promoter regions. Thirteen of twenty-two tested gene promoters were methylated in cell lines, and ten in primary ESCC accompanied by silencing at the mRNA level. Potent growth suppressive activity of three genes including CRIP-1, Apolipoprotein D, and Neuromedin U in ESCC cells was demonstrated by colony focus assays. Pharmacologic reversal of epigenetic silencing is a powerful approach for comprehensive identification of tumor suppressor genes in human cancers.

本文言語英語
ページ(範囲)485-495
ページ数11
ジャーナルCancer Cell
2
6
DOI
出版ステータス出版済み - 12月 2002
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 腫瘍学
  • 細胞生物学
  • 癌研究

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