Pharmacological targeting of HSP90 with 17-AAG induces apoptosis of myogenic cells through activation of the intrinsic pathway

Akira Wagatsuma, Yuzo Takayama, Takayuki Hoshino, Masataka Shiozuka, Shigeru Yamada, Ryoichi Matsuda, Kunihiko Mabuchi

研究成果: Contribution to journalArticle査読

9 被引用数 (Scopus)

抄録

We have shown that pharmacological inhibition of HSP90 ATPase activity induces apoptosis of myoblasts during their differentiation. However, the signaling pathways remain not fully characterized. We report that pharmacological targeting of HSP90 with 17-AAG activates the intrinsic pathway including caspase-dependent and caspase-independent pathways. 17-AAG induces the typical apoptotic phenotypes including PARP cleavage, chromatin condensation, and nuclear fragmentation with mitochondrial release of cytochrome c, Smac/DIABLO, procaspase-9 processing, and caspase-3 activation. AIF and EndoG redistribute from the mitochondria into the cytosol and are partially translocated to the nucleus in 17-AAG-treated cells. These results suggest that caspase-dependent and caspase-independent pathways should be considered in apoptosis of myogenic cells induced by inhibition of HSP90 ATPase activity.

本文言語英語
ページ(範囲)45-58
ページ数14
ジャーナルMolecular and cellular biochemistry
445
1-2
DOI
出版ステータス出版済み - 8 1 2018

All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 臨床生化学
  • 細胞生物学

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