Phase separation multi-phase flow using an aqueous two-phase system of a polyethylene glycol/dextran mixed solution

Naoya Imanishi, Tetsuo Yamasaki, Kazuhiko Tsukagoshi, Masaharu Murata

研究成果: Contribution to journalArticle査読

2 被引用数 (Scopus)

抄録

Polyethylene glycol/dextran mixed solution as an aqueous two-phase system was fed into a fused-silica capillary tube under different conditions, resulting in phase transformation leading to phase separation multi-phase flow through/along a liquid-liquid interface. As one flow-type example, when 6.4 wt% polyethylene glycol and 9.7 wt% dextran aqueous solution containing 1.0 mM Rhodamine B was fed into the capillary tube at 3°C, tube radial distribution flow (annual flow) was observed through bright-field microscopy. Tube radial distribution flow consisted of a dextran-rich inner phase and polyethylene glycol-rich outer phase. We also examined the distribution of proteins, such as bovine serum albumin, hemoglobin, and lysozyme, in the inner and outer phases through use of double capillary tubes with different inner diameters. The protein distribution was greater in the inner (dextran-rich) phase than the outer (polyethylene glycol-rich) phase. The distribution ratios of the three proteins (ratio of the inner/outer protein concentration) were 2.3, 4.2, and 1.8, respectively. The proteins concentrated in the dextran-rich phase through tube radial distribution flow of a polyethylene glycol/dextran mixed solution.

本文言語英語
ページ(範囲)953-958
ページ数6
ジャーナルanalytical sciences
34
8
DOI
出版ステータス出版済み - 2018

All Science Journal Classification (ASJC) codes

  • 分析化学

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