Phenol-Sulfate Conjugating Activity in Liver-Soluble Fraction of Fish

Shigeo Kimura, Yuji Oshima, Kunio Kobayashi

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

The phenol-sulfate conjugation by the liver subcellular fractions from goldfish Carassius auratus, carp Cyprinus carpio, rainbow trout Salmo gairdneri, and Tilapia nilotica has been studied using [35S] K2SO4. The cell fractionation of goldfish liver was examined by the determination of respective markers for nuclear, mitochondrial, lysosomal, microsomal, and soluble fractions. Among those subcellular fractions, only the soluble fraction displayed the conjugating activity. The maximum activity of the enzyme in the soluble fraction was obtained in the presence of 20 µmol of both ATP and Mg2+ in 1.5 ml of medium, although the activity decreased with an excess of ATP and Mg2+. This conjugation was inhibited by more than 0.1 µmol of Ca2+ in 1.5 ml of medium. The enzyme exhibited the conjugating activity in the pH range 7.5–9.5; the optimum value was at 8. In the temperature range 5–40°C, the maximum conjugation in goldfish, Tilapia, and carp occurred at 35°C, in rainbow trout it occurred at 30°C. The phenol-sulfate conjugating activities in goldfish, carp, rainbow trout, Tilapia and albino rat were approximately 340, 82, 66, 200 and 720 nmol/g-liver per h, respectively.

元の言語英語
ページ(範囲)433-437
ページ数5
ジャーナルNIPPON SUISAN GAKKAISHI
48
発行部数3
DOI
出版物ステータス出版済み - 1 1 1982

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goldfish
phenol
rainbow
Oncorhynchus mykiss
sulfates
carp
sulfate
subcellular fractions
liver
tilapia (common name)
fish
enzyme
cell fractionation
Carassius auratus
Oreochromis niloticus
fractionation
Cyprinus carpio
albino
enzyme activity
calcium

All Science Journal Classification (ASJC) codes

  • Aquatic Science

これを引用

Phenol-Sulfate Conjugating Activity in Liver-Soluble Fraction of Fish. / Kimura, Shigeo; Oshima, Yuji; Kobayashi, Kunio.

:: NIPPON SUISAN GAKKAISHI, 巻 48, 番号 3, 01.01.1982, p. 433-437.

研究成果: ジャーナルへの寄稿記事

Kimura, Shigeo ; Oshima, Yuji ; Kobayashi, Kunio. / Phenol-Sulfate Conjugating Activity in Liver-Soluble Fraction of Fish. :: NIPPON SUISAN GAKKAISHI. 1982 ; 巻 48, 番号 3. pp. 433-437.
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