TY - JOUR
T1 - Phenotypic characterization and virulence-related properties of Escherichia albertii strains isolated from children with diarrhea in Brazil
AU - Lima, Mauricio P.
AU - Yamamoto, Denise
AU - de Mello Santos, Ana Carolina
AU - Ooka, Tadasuke
AU - Hernandes, Rodrigo T.
AU - Vieira, Mônica A.M.
AU - Santos, Fernanda Fernandes
AU - Silva, Rosa Maria
AU - Hayashi, Tetsuya
AU - Gomes, Tânia A.T.
N1 - Funding Information:
This work was supported by grants from Fundac¸ão de Amparo à Pesquisa do Estado de São Paulo [FAPESP 2011/12664–5] to TATG, JSPS KAKENHI [grant 16K08781] to TO, and the Japan Agency for Medical Research and Development (AMED) [JP17fk0108308j0003] to TH. This study was financed in part by the Coordenac¸ão de Aperfeic¸oamento de Pessoal de Nível Superior—Brasil with scholarships to MPL (CAPES Finance Code 001), to TATG and DY CAPES [AUX-PE-PNPD 2350/2011], and to ACMS (CAPES [AUX-PE-PNPD 001]). FFS received a scholarship from Conselho Nacional de Pesquisa (CNPq) [140 443/2014–2].
Publisher Copyright:
© FEMS 2019. All rights reserved.
PY - 2019/3/1
Y1 - 2019/3/1
N2 - Escherichia albertii are emerging enteropathogens, whose identification is difficult, as they share biochemical characteristics and some virulence-related genes with diarrheagenic Escherichia coli (DEC). Studies on phylogeny, phenotypic characteristics and potential virulence factors of human E. albertii strains are scarce. In this study, we identified by multiplex PCR five E. albertii among 106 strains isolated from diarrheic children in São Paulo, Brazil, which were previously classified as atypical enteropathogenic E. coli. All strains were investigated regarding their phylogeny, biochemical properties, virulence-related properties, antimicrobial resistance and presence of putative virulence-related genes. All strains belonged to different E. albertii lineages and adhered to and produced attaching and effacing lesions on HeLa cells. Three strains invaded Caco-2 cells, but did not persist intracellularly, and three formed biofilms on polystyrene surfaces. All strains were resistant to few antibiotics and only one carried a self-transmissible resistance plasmid. Finally, among 38 DEC and 18 extraintestinal pathogenic E. coli (ExPEC) virulence-related genes searched, six and three were detected, respectively, with paa and cdtB being found in all strains. Despite the limited number of strains, this study provided additional knowledge on human E. albertii virulence potential, showing that they share important virulence factors with DEC and ExPEC.
AB - Escherichia albertii are emerging enteropathogens, whose identification is difficult, as they share biochemical characteristics and some virulence-related genes with diarrheagenic Escherichia coli (DEC). Studies on phylogeny, phenotypic characteristics and potential virulence factors of human E. albertii strains are scarce. In this study, we identified by multiplex PCR five E. albertii among 106 strains isolated from diarrheic children in São Paulo, Brazil, which were previously classified as atypical enteropathogenic E. coli. All strains were investigated regarding their phylogeny, biochemical properties, virulence-related properties, antimicrobial resistance and presence of putative virulence-related genes. All strains belonged to different E. albertii lineages and adhered to and produced attaching and effacing lesions on HeLa cells. Three strains invaded Caco-2 cells, but did not persist intracellularly, and three formed biofilms on polystyrene surfaces. All strains were resistant to few antibiotics and only one carried a self-transmissible resistance plasmid. Finally, among 38 DEC and 18 extraintestinal pathogenic E. coli (ExPEC) virulence-related genes searched, six and three were detected, respectively, with paa and cdtB being found in all strains. Despite the limited number of strains, this study provided additional knowledge on human E. albertii virulence potential, showing that they share important virulence factors with DEC and ExPEC.
UR - http://www.scopus.com/inward/record.url?scp=85064950486&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85064950486&partnerID=8YFLogxK
U2 - 10.1093/femspd/ftz014
DO - 10.1093/femspd/ftz014
M3 - Article
C2 - 30865776
AN - SCOPUS:85064950486
VL - 77
JO - Pathogens and Disease
JF - Pathogens and Disease
SN - 2049-632X
IS - 2
ER -
