Phenotypic instability of transgenic tobacco plants and their progenies expressing Arabidopsis thafiana small GTP-binding protein genes

Evalour T. Aspuria, Toyoaki Anai, Nobuharu Fujii, Takashi Ueda, Masahiro Miyoshi, Minami Matsui, Hirofumi Uchimiya

研究成果: Contribution to journalArticle査読

10 被引用数 (Scopus)

抄録

Chimeric genes consisting of the cauliflower mosaic virus 35S promoter, a CDNA encoding a small GTP-binding protein from Arabidopsis thaliana (ara-2 or ara-4) and the terminator of the nopaline synthase gene were cloned into a binary vector. Tobacco leaf tissues were transformed with this plasmid via Agrobacterium-mediated transformation. Transgenic plants possessing either ara-2 or ara-4 occasionally showed morphological abnormalities in leaves and other organs. However, such alterations were not always associated with co-transferred characters, such as kanamycin tolerance, and they arose in no more than 10% of the transgenic plants. Such phenomena were also observed in the progenies of the primary transgenic plants. Despite such unusual inheritance of the phenotypic abnormalities, GTP-binding activity of the inserted ara gene products was detected in all plants tested.

本文言語英語
ページ(範囲)509-513
ページ数5
ジャーナルMolecular and General Genetics
246
4
DOI
出版ステータス出版済み - 7 1995
外部発表はい

All Science Journal Classification (ASJC) codes

  • 遺伝学

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