抄録
The VPS34 gene product (Vps34p) is required for protein sorting to the lysosome-like vacuole of the yeast Saccharomyces cerevisiae. Vps34p shares significant sequence similarity with the catalytic subunit of bovine phosphatidylinositol (PI) 3-kinase [the 110-kilodalton (p110) subunit of PI 3-kinase], which is known to interact with activated cell surface receptor tyrosine kinases. Yeast strains deleted for the VPS34 gene or carrying vps34 point mutations lacked detectable PI 3-kinase activity and exhibited severe defects in vacuolar protein sorting. Overexpression of Vps34p resulted in an increase in PI 3-kinase activity, and this activity was specifically precipitated with antisera to Vps34p. VPS34 encodes a yeast PI 3-kinase, and this enzyme appears to regulate intracellular protein trafficking decisions.
本文言語 | 英語 |
---|---|
ページ(範囲) | 88-91 |
ページ数 | 4 |
ジャーナル | Science |
巻 | 260 |
号 | 5104 |
DOI | |
出版ステータス | 出版済み - 1993 |
外部発表 | はい |
!!!All Science Journal Classification (ASJC) codes
- 一般