Phosphatidylserine transport by Ups2-Mdm35 in respiration-active mitochondria

Non Miyata, Yasunori Watanabe, Yasushi Tamura, Toshiya Endo, Osamu Kuge

研究成果: ジャーナルへの寄稿記事

26 引用 (Scopus)

抄録

Phosphatidylethanolamine (PE) is an essential phospholipid for mitochondrial functions and is synthesized mainly by phosphatidylserine (PS) decarboxylase at the mitochondrial inner membrane. In Saccharomyces cerevisiae, PS is synthesized in the endoplasmic reticulum (ER), such that mitochondrial PE synthesis requires PS transport from the ER to the mitochondrial inner membrane. Here, we provide evidence that Ups2-Mdm35, a protein complex localized at the mitochondrial intermembrane space, mediates PS transport for PE synthesis in respiration-active mitochondria. UPS2- and MDM35-null mutations greatly attenuated conversion of PS to PE in yeast cells growing logarithmically under nonfermentable conditions, but not fermentable conditions. A recombinant Ups2-Mdm35 fusion protein exhibited phospholipidtransfer activity between liposomes in vitro. Furthermore, UPS2 expression was elevated under nonfermentable conditions and at the diauxic shift, the metabolic transition from glycolysis to oxidative phosphorylation. These results demonstrate that Ups2-Mdm35 functions as a PS transfer protein and enhances mitochondrial PE synthesis in response to the cellular metabolic state.

元の言語英語
ページ(範囲)77-88
ページ数12
ジャーナルJournal of Cell Biology
214
発行部数1
DOI
出版物ステータス出版済み - 7 1 2016

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Phosphatidylserines
Mitochondria
Respiration
Mitochondrial Membranes
Endoplasmic Reticulum
Mitochondrial Proteins
Oxidative Phosphorylation
Glycolysis
Liposomes
Saccharomyces cerevisiae
Proteins
Yeasts
phosphatidylethanolamine
Mutation

All Science Journal Classification (ASJC) codes

  • Cell Biology

これを引用

Phosphatidylserine transport by Ups2-Mdm35 in respiration-active mitochondria. / Miyata, Non; Watanabe, Yasunori; Tamura, Yasushi; Endo, Toshiya; Kuge, Osamu.

:: Journal of Cell Biology, 巻 214, 番号 1, 01.07.2016, p. 77-88.

研究成果: ジャーナルへの寄稿記事

Miyata, Non ; Watanabe, Yasunori ; Tamura, Yasushi ; Endo, Toshiya ; Kuge, Osamu. / Phosphatidylserine transport by Ups2-Mdm35 in respiration-active mitochondria. :: Journal of Cell Biology. 2016 ; 巻 214, 番号 1. pp. 77-88.
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