Phosphorylation of mitogen-activated protein kinase cascade during early embryo development in the mouse

N. Iwamori, K. Naito, K. Sugiura, H. Kagii, M. Yamashita, S. Ohashi, S. Goto, K. Yamanouchi, H. Tojo

研究成果: ジャーナルへの寄稿学術誌査読

7 被引用数 (Scopus)

抄録

The mitogen-activated protein kinase (MAPK) cascade is one of the most important signal transduction pathways that regulate the cell cycle in somatic cells. The present study examined the phosphorylation states of components in the MAPK cascade, Raf-1, MEK-1, and extracellular signal regulated kinases (ERKs), which are activated by mitogens, throughout early mouse embryo development and in cultured somatic cells generally. In somatic cells, Raf-1 and MEK-1 were phosphorylated at M-phase and dephosphorylated during interphase. ERKs were not phosphorylated at any stage during the cell cycle. These results were similar to previous findings for the first and second cell cycles of early mouse embryos. In contrast, after the four-cell stage, not only ERKs, but also Raf-1 and MEK-1, were not phosphorylated at any stage during the cell cycle in mouse early embryos. These results suggest that the MAPK cascade in mouse-embryos is regulated by the same mechanism as in somatic cells before the two-cell stage, and that regulation is changed to an embryo-specific mechanism after the four-cell stage.

本文言語英語
ページ(範囲)209-214
ページ数6
ジャーナルReproduction, Fertility and Development
12
3-4
DOI
出版ステータス出版済み - 2000
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • 生殖医学
  • 動物科学および動物学
  • 分子生物学
  • 遺伝学
  • 内分泌学
  • 発生生物学

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