Phosphorylation of STAT3 in undifferentiated pleomorphic sarcoma is correlated with a favorable prognosis

Hirofumi Bekki, Kenichi Kouhashi, Yuichi Yamada, Kunio Iura, Takeaki Ishii, Akira Maekawa, Hiroshi Otsuka, Hidetaka Yamamoto, Michiyuki Hakozaki, Kazuki Nabeshima, Yukihide Iwamoto, Yoshinao Oda

研究成果: ジャーナルへの寄稿記事

5 引用 (Scopus)

抄録

Objective: The Janus kinase (JAK)-signal transducer and activator of transcription (STAT) pathway plays a role in various biological processes. Phosphorylated STAT3 (p-STAT3) functions as a transcriptional factor, and suppressor of cytokine signaling 3 (SOCS3) is a potential inhibitor of STAT3. Here, we analyzed the status of the JAK-STAT pathway in undifferentiated pleomorphic sarcoma (UPS). Methods: We performed immunohistochemistry in 79 samples of UPS and Western blotting in 10 frozen samples. We also examined alterations in protein expression in the JAK-STAT pathway after the inhibition of phosphorylated Akt (p-Akt) or extracellular signal-regulated kinase (p-Erk) in vitro. Results: Immunohistochemically, p-STAT3 and SOCS3 were positive in 59.7 and 55.8%, respectively. Positivity for p-STAT3 was significantly correlated with a better prognosis (p = 0.0006) and negatively with SOCS3 expression (p = 0.0223). Positivity for SOCS3 was significantly correlated with a worse prognosis (p = 0.0001). Western blotting analysis revealed that p-STAT3 expression was lower in tumor than in normal tissue. In vitro results demonstrated that there was no detectable change in the expression of p-STAT3 regardless of the status of p-Akt or p-Erk. Conclusion: P-STAT3 may be a useful prognostic factor for UPS.

元の言語英語
ページ(範囲)161-169
ページ数9
ジャーナルPathobiology
84
発行部数3
DOI
出版物ステータス出版済み - 5 1 2017

Fingerprint

Janus Kinases
Sarcoma
Transducers
Phosphorylation
Cytokines
Extracellular Signal-Regulated MAP Kinases
Western Blotting
Biological Phenomena
Immunohistochemistry
Neoplasms
Proteins
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

これを引用

Phosphorylation of STAT3 in undifferentiated pleomorphic sarcoma is correlated with a favorable prognosis. / Bekki, Hirofumi; Kouhashi, Kenichi; Yamada, Yuichi; Iura, Kunio; Ishii, Takeaki; Maekawa, Akira; Otsuka, Hiroshi; Yamamoto, Hidetaka; Hakozaki, Michiyuki; Nabeshima, Kazuki; Iwamoto, Yukihide; Oda, Yoshinao.

:: Pathobiology, 巻 84, 番号 3, 01.05.2017, p. 161-169.

研究成果: ジャーナルへの寄稿記事

Bekki, H, Kouhashi, K, Yamada, Y, Iura, K, Ishii, T, Maekawa, A, Otsuka, H, Yamamoto, H, Hakozaki, M, Nabeshima, K, Iwamoto, Y & Oda, Y 2017, 'Phosphorylation of STAT3 in undifferentiated pleomorphic sarcoma is correlated with a favorable prognosis', Pathobiology, 巻. 84, 番号 3, pp. 161-169. https://doi.org/10.1159/000448524
Bekki, Hirofumi ; Kouhashi, Kenichi ; Yamada, Yuichi ; Iura, Kunio ; Ishii, Takeaki ; Maekawa, Akira ; Otsuka, Hiroshi ; Yamamoto, Hidetaka ; Hakozaki, Michiyuki ; Nabeshima, Kazuki ; Iwamoto, Yukihide ; Oda, Yoshinao. / Phosphorylation of STAT3 in undifferentiated pleomorphic sarcoma is correlated with a favorable prognosis. :: Pathobiology. 2017 ; 巻 84, 番号 3. pp. 161-169.
@article{fe4d477eb5994a8b98ba46c234020034,
title = "Phosphorylation of STAT3 in undifferentiated pleomorphic sarcoma is correlated with a favorable prognosis",
abstract = "Objective: The Janus kinase (JAK)-signal transducer and activator of transcription (STAT) pathway plays a role in various biological processes. Phosphorylated STAT3 (p-STAT3) functions as a transcriptional factor, and suppressor of cytokine signaling 3 (SOCS3) is a potential inhibitor of STAT3. Here, we analyzed the status of the JAK-STAT pathway in undifferentiated pleomorphic sarcoma (UPS). Methods: We performed immunohistochemistry in 79 samples of UPS and Western blotting in 10 frozen samples. We also examined alterations in protein expression in the JAK-STAT pathway after the inhibition of phosphorylated Akt (p-Akt) or extracellular signal-regulated kinase (p-Erk) in vitro. Results: Immunohistochemically, p-STAT3 and SOCS3 were positive in 59.7 and 55.8{\%}, respectively. Positivity for p-STAT3 was significantly correlated with a better prognosis (p = 0.0006) and negatively with SOCS3 expression (p = 0.0223). Positivity for SOCS3 was significantly correlated with a worse prognosis (p = 0.0001). Western blotting analysis revealed that p-STAT3 expression was lower in tumor than in normal tissue. In vitro results demonstrated that there was no detectable change in the expression of p-STAT3 regardless of the status of p-Akt or p-Erk. Conclusion: P-STAT3 may be a useful prognostic factor for UPS.",
author = "Hirofumi Bekki and Kenichi Kouhashi and Yuichi Yamada and Kunio Iura and Takeaki Ishii and Akira Maekawa and Hiroshi Otsuka and Hidetaka Yamamoto and Michiyuki Hakozaki and Kazuki Nabeshima and Yukihide Iwamoto and Yoshinao Oda",
year = "2017",
month = "5",
day = "1",
doi = "10.1159/000448524",
language = "English",
volume = "84",
pages = "161--169",
journal = "Pathobiology",
issn = "1015-2008",
publisher = "S. Karger AG",
number = "3",

}

TY - JOUR

T1 - Phosphorylation of STAT3 in undifferentiated pleomorphic sarcoma is correlated with a favorable prognosis

AU - Bekki, Hirofumi

AU - Kouhashi, Kenichi

AU - Yamada, Yuichi

AU - Iura, Kunio

AU - Ishii, Takeaki

AU - Maekawa, Akira

AU - Otsuka, Hiroshi

AU - Yamamoto, Hidetaka

AU - Hakozaki, Michiyuki

AU - Nabeshima, Kazuki

AU - Iwamoto, Yukihide

AU - Oda, Yoshinao

PY - 2017/5/1

Y1 - 2017/5/1

N2 - Objective: The Janus kinase (JAK)-signal transducer and activator of transcription (STAT) pathway plays a role in various biological processes. Phosphorylated STAT3 (p-STAT3) functions as a transcriptional factor, and suppressor of cytokine signaling 3 (SOCS3) is a potential inhibitor of STAT3. Here, we analyzed the status of the JAK-STAT pathway in undifferentiated pleomorphic sarcoma (UPS). Methods: We performed immunohistochemistry in 79 samples of UPS and Western blotting in 10 frozen samples. We also examined alterations in protein expression in the JAK-STAT pathway after the inhibition of phosphorylated Akt (p-Akt) or extracellular signal-regulated kinase (p-Erk) in vitro. Results: Immunohistochemically, p-STAT3 and SOCS3 were positive in 59.7 and 55.8%, respectively. Positivity for p-STAT3 was significantly correlated with a better prognosis (p = 0.0006) and negatively with SOCS3 expression (p = 0.0223). Positivity for SOCS3 was significantly correlated with a worse prognosis (p = 0.0001). Western blotting analysis revealed that p-STAT3 expression was lower in tumor than in normal tissue. In vitro results demonstrated that there was no detectable change in the expression of p-STAT3 regardless of the status of p-Akt or p-Erk. Conclusion: P-STAT3 may be a useful prognostic factor for UPS.

AB - Objective: The Janus kinase (JAK)-signal transducer and activator of transcription (STAT) pathway plays a role in various biological processes. Phosphorylated STAT3 (p-STAT3) functions as a transcriptional factor, and suppressor of cytokine signaling 3 (SOCS3) is a potential inhibitor of STAT3. Here, we analyzed the status of the JAK-STAT pathway in undifferentiated pleomorphic sarcoma (UPS). Methods: We performed immunohistochemistry in 79 samples of UPS and Western blotting in 10 frozen samples. We also examined alterations in protein expression in the JAK-STAT pathway after the inhibition of phosphorylated Akt (p-Akt) or extracellular signal-regulated kinase (p-Erk) in vitro. Results: Immunohistochemically, p-STAT3 and SOCS3 were positive in 59.7 and 55.8%, respectively. Positivity for p-STAT3 was significantly correlated with a better prognosis (p = 0.0006) and negatively with SOCS3 expression (p = 0.0223). Positivity for SOCS3 was significantly correlated with a worse prognosis (p = 0.0001). Western blotting analysis revealed that p-STAT3 expression was lower in tumor than in normal tissue. In vitro results demonstrated that there was no detectable change in the expression of p-STAT3 regardless of the status of p-Akt or p-Erk. Conclusion: P-STAT3 may be a useful prognostic factor for UPS.

UR - http://www.scopus.com/inward/record.url?scp=84988583252&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84988583252&partnerID=8YFLogxK

U2 - 10.1159/000448524

DO - 10.1159/000448524

M3 - Article

VL - 84

SP - 161

EP - 169

JO - Pathobiology

JF - Pathobiology

SN - 1015-2008

IS - 3

ER -