Phosphorylation signal detection on a peptide chip

Shuhei Shigaki, Xiaoming Han, Takayuki Yamaji, Go Yamanouchi, Takeshi Mori, Takuro Nidome, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

抄録

An intracellular signal transduction system involved in many kinds of proteins regulates various cellular events, such as cellular proliferation, differentiation and cell death, responding to the outer environment. Among those proteins, protein kinases are one of the most important class of proteins. It has been reported that abnormal activities of protein kinases are related to various diseases. Therefore, an analysis of protein kinase activity would be useful for diagnosis and drug screening. Herein, we describe the detection of on-chip phosphorylation using a peptide chip by fluorescence imaging. In this work, we selected a cAMP-dependent protein kinase (PKA) as a model, and quantitatively evaluated the substrate specificity for PKA using a peptide chip. Moreover, we attempted to detect intracellular kinase activity. Since src correlates with breast cancer, we measured the src activity in MCF-7 human breast cancer cell lysate. Consequently, with our peptide chip we could detect the src activity in MCF-7 cell lysate. Therefore, this method could be applied to diagnosis, drug discovery and screening.

元の言語英語
ページ(範囲)969-973
ページ数5
ジャーナルbunseki kagaku
55
発行部数12
DOI
出版物ステータス出版済み - 12 1 2006

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Phosphorylation
Signal detection
Protein Kinases
Peptides
Screening
Signal transduction
Proteins
Cell death
Cyclic AMP-Dependent Protein Kinases
Phosphotransferases
Fluorescence
Cells
Imaging techniques
Substrates
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

これを引用

Shigaki, S., Han, X., Yamaji, T., Yamanouchi, G., Mori, T., Nidome, T., & Katayama, Y. (2006). Phosphorylation signal detection on a peptide chip. bunseki kagaku, 55(12), 969-973. https://doi.org/10.2116/bunsekikagaku.55.969

Phosphorylation signal detection on a peptide chip. / Shigaki, Shuhei; Han, Xiaoming; Yamaji, Takayuki; Yamanouchi, Go; Mori, Takeshi; Nidome, Takuro; Katayama, Yoshiki.

:: bunseki kagaku, 巻 55, 番号 12, 01.12.2006, p. 969-973.

研究成果: ジャーナルへの寄稿記事

Shigaki, S, Han, X, Yamaji, T, Yamanouchi, G, Mori, T, Nidome, T & Katayama, Y 2006, 'Phosphorylation signal detection on a peptide chip', bunseki kagaku, 巻. 55, 番号 12, pp. 969-973. https://doi.org/10.2116/bunsekikagaku.55.969
Shigaki S, Han X, Yamaji T, Yamanouchi G, Mori T, Nidome T その他. Phosphorylation signal detection on a peptide chip. bunseki kagaku. 2006 12 1;55(12):969-973. https://doi.org/10.2116/bunsekikagaku.55.969
Shigaki, Shuhei ; Han, Xiaoming ; Yamaji, Takayuki ; Yamanouchi, Go ; Mori, Takeshi ; Nidome, Takuro ; Katayama, Yoshiki. / Phosphorylation signal detection on a peptide chip. :: bunseki kagaku. 2006 ; 巻 55, 番号 12. pp. 969-973.
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