Photoactive, covalent attachment of deoxyribonucleic acid on gold with double-strand specificity using self-assembled monolayers containing psoralen

Koji Nakano, Hideshi Matsunaga, Keisuke Sai, Nobuaki Soh, Toshihiko Imato

    研究成果: Contribution to journalArticle査読

    12 被引用数 (Scopus)

    抄録

    Taking advantages of psoralen photochemistry, we have developed a new method of immobilizing DNA on gold substrate surfaces. A psoralen derivative having an alkylamine function was synthesized, and was self-assembled on gold substrate surfaces in a combined use of a thiol-derivatized molecule, 3,3′-dithiobis(succinimidyl propionate) forming amide bonds on the surface. We found that by irradiating with long wavelength ultraviolet light (320-400 nm), DNA molecules added in the solution phase were covalently immobilized on the monolayer surface through the photoadduct formation of the psoralen molecules with the DNA nucleobases. The present method has its advantage that is applicable to native DNAs, no chemically modifying DNAs, in spite of its covalent immobilization principle. We have examined 12 mer synthetic oligonucleotide immobilizations and have found that the surface concentration thus attained was to be 20 pmol cm-2, which is consistent with saturated surface coverage. Interestingly, the immobilization occurred double-stranded-DNA-preferentially; no immobilization for single-stranded DNAs. Characterization of the immobilization chemistry has been achieved using atomic force microscopic imaging, infrared absorption, X-ray photoelectron spectroscopy, electrochemistry, and quartz-crystal microbalance and their results were described.

    本文言語英語
    ページ(範囲)93-99
    ページ数7
    ジャーナルAnalytica Chimica Acta
    578
    1
    DOI
    出版ステータス出版済み - 9 18 2006

    All Science Journal Classification (ASJC) codes

    • 分析化学
    • 生化学
    • 環境化学
    • 分光学

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