Photocatalytic nanofabrication and intracellular raman imaging of living cells with functionalized AFM probes

Takayuki Shibata, Hiromi Furukawa, Yasuharu Ito, Masahiro Nagahama, Terutake Hayashi, Miho Ishii-Teshima, Moeto Nagai

研究成果: ジャーナルへの寄稿学術誌査読

4 被引用数 (Scopus)


Atomic force microscopy (AFM) is an effective platform for in vitro manipulation and analysis of living cells in medical and biological sciences. To introduce additional new features and functionalities into a conventional AFM system, we investigated the photocatalytic nanofabrication and intracellular Raman imaging of living cells by employing functionalized AFM probes. Herein, we investigated the effect of indentation speed on the cell membrane perforation of living HeLa cells based on highly localized photochemical oxidation with a catalytic titanium dioxide (TiO2)-functionalized AFM probe. On the basis of force-distance curves obtained during the indentation process, the probability of cell membrane perforation, penetration force, and cell viability was determined quantitatively. Moreover, we explored the possibility of intracellular tip-enhanced Raman spectroscopy (TERS) imaging of molecular dynamics in living cells via an AFM probe functionalized with silver nanoparticles in a homemade Raman system integrated with an inverted microscope. We successfully demonstrated that the intracellular TERS imaging has the potential to visualize distinctly different features in Raman spectra between the nucleus and the cytoplasm of a single living cell and to analyze the dynamic behavior of biomolecules inside a living cell.

出版ステータス出版済み - 5月 1 2020

!!!All Science Journal Classification (ASJC) codes

  • 制御およびシステム工学
  • 機械工学
  • 電子工学および電気工学


「Photocatalytic nanofabrication and intracellular raman imaging of living cells with functionalized AFM probes」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。