Phylogenetic position and peculiar genetic traits of a midgut bacterial symbiont of the stinkbug parastrachia japonensis

Takahiro Hosokawa, Yoshitomo Kikuchi, Naruo Nikon, Xian Ying Meng, Mantaro Hironaka, Takema Fukatsu

研究成果: Contribution to journalArticle査読

38 被引用数 (Scopus)

抄録

The stinkbug Parastrachia japonensis (Hemiptera: Parastrachiidae) is known for its prolonged prereproductive nonfeeding period, maternal care of eggs in an underground nest, and maternal collection and provisioning of food (fruits) for nymphs. A previous study suggested that a bacterial symbiont is involved in uric acid recycling in this insect during the nonfeeding period, but the identity of this symbiont has not been determined. Here we characterized a novel bacterial symbiont obtained from P. japonensis. Molecular phylogenetic analyses based on 16S rRNA, gyrB, and groEL gene sequences consistently indicated that this symbiont constituted a distinct lineage in the Gammaproteobacteria that has no close relatives but is allied with gut symbionts of acanthosomatid and plataspid stinkbugs, as well as with endocellular symbionts of sharpshooters, tsetse flies, and aphids. The symbiont genes had a remarkably AT-biased nucleotide composition and exhibited significantly accelerated molecular evolution. The symbiont genome was extremely reduced; its size was estimated to be 0.85 Mb. These results suggest that there has been an intimate host-symbiont association over evolutionary time. The symbiont was localized in swollen crypts in a posterior part of the midgut, which was a specialized symbiotic organ. The possibility that the symbiont is involved in uric acid recycling is discussed. The designation "Candidatus Benitsuchiphilus tojoi" is proposed for the symbiont.

本文言語英語
ページ(範囲)4130-4135
ページ数6
ジャーナルApplied and environmental microbiology
76
13
DOI
出版ステータス出版済み - 7 2010
外部発表はい

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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