Phylogenetic relationships among tea cultivars based on AFLP analysis

Seonha Lee, Jungho Kim, Junichi Sano, Yukio Ozaki, Hiroshi Okubo

研究成果: ジャーナルへの寄稿記事

7 引用 (Scopus)

抄録

Several modifications of the AFLP protocol were made to simplify the analysis, i.e., the amount of the reagents for restriction digestion, ligation of adaptors, preamplification and selective amplification, the primers without labeling, and the gel staining with ethidium bromide. The phylogenetic relationships among 37 accessions of the genus Camellia were assessed using the modified AFLP technique and cluster analysis. The accessions, 24 Japanese tea cultivars, seven Korean wild tea varieties and six Camellia species closely related to tea, were clustered into five groups; Japanese tea cultivars closely related to 'Yabukita', those not closely related to 'Yabukita', Chinese tea cultivars and Korean wild tea varieties, Assam type black tea cultivars, and Camellia species closely related to tea. The results of this study indicated that the morphological characteristics and the origins of the tea cultivars were well reflected in the clustering of the cultivars based on the AFLP analysis, and that the AFLP technique might find its use as an efficient and effective tool for determining phylogenetic relationships among tea cultivars.

元の言語英語
ページ(範囲)289-299
ページ数11
ジャーナルJournal of the Faculty of Agriculture, Kyushu University
47
発行部数2
出版物ステータス出版済み - 2 1 2003

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Amplified Fragment Length Polymorphism Analysis
Tea
tea
amplified fragment length polymorphism
phylogeny
cultivars
Camellia
Cluster Analysis
black tea
Ethidium
cluster analysis
Ligation
Digestion
digestion
gels
Gels
India

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

これを引用

Phylogenetic relationships among tea cultivars based on AFLP analysis. / Lee, Seonha; Kim, Jungho; Sano, Junichi; Ozaki, Yukio; Okubo, Hiroshi.

:: Journal of the Faculty of Agriculture, Kyushu University, 巻 47, 番号 2, 01.02.2003, p. 289-299.

研究成果: ジャーナルへの寄稿記事

Lee, Seonha ; Kim, Jungho ; Sano, Junichi ; Ozaki, Yukio ; Okubo, Hiroshi. / Phylogenetic relationships among tea cultivars based on AFLP analysis. :: Journal of the Faculty of Agriculture, Kyushu University. 2003 ; 巻 47, 番号 2. pp. 289-299.
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