抄録
WRN interacting protein 1 (WRNIP1) was originally identified as a protein that interacts with the Werner syndrome responsible gene product (WRN). WRNIP1 is a highly conserved protein from E. coli to humans. Genetic studies in budding yeast suggested that the yeast orthlog of WRNIP1, Mgs1, may function in a DNA damage tolerance pathway that is similar to, but distinct from, the template-switch damage avoidance pathway involving Rad6, Rad18, Rad5, Mms2, and Ubc13. Here we report that human WRNIP1 binds in an ATP dependent manner to both forked DNA that mimics stalled replication forks and to template/primer DNA. We found that WRNIP1 interacts physically with RAD18 and interferes with the binding of RAD18 to forked DNA and to template/primer DNA. In contrast, RAD18 enhances the binding of WRNIP1 to these DNAs, suggesting that WRNIP1 targets DNA bound by RAD18.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 171-178 |
| ページ数 | 8 |
| ジャーナル | Genes and Genetic Systems |
| 巻 | 84 |
| 発行部数 | 2 |
| DOI | |
| 出版物ステータス | 出版済み - 7 14 2009 |
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All Science Journal Classification (ASJC) codes
- Molecular Biology
- Genetics
これを引用
Physical and functional interaction between WRNIP1 and RAD18. / Yoshimura, Akari; Seki, Masayuki; Kanamori, Makoto; Tateishi, Satoshi; Tsurimoto, Toshiki; Tada, Shusuke; Enomoto, Takemi.
：: Genes and Genetic Systems, 巻 84, 番号 2, 14.07.2009, p. 171-178.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Physical and functional interaction between WRNIP1 and RAD18
AU - Yoshimura, Akari
AU - Seki, Masayuki
AU - Kanamori, Makoto
AU - Tateishi, Satoshi
AU - Tsurimoto, Toshiki
AU - Tada, Shusuke
AU - Enomoto, Takemi
PY - 2009/7/14
Y1 - 2009/7/14
N2 - WRN interacting protein 1 (WRNIP1) was originally identified as a protein that interacts with the Werner syndrome responsible gene product (WRN). WRNIP1 is a highly conserved protein from E. coli to humans. Genetic studies in budding yeast suggested that the yeast orthlog of WRNIP1, Mgs1, may function in a DNA damage tolerance pathway that is similar to, but distinct from, the template-switch damage avoidance pathway involving Rad6, Rad18, Rad5, Mms2, and Ubc13. Here we report that human WRNIP1 binds in an ATP dependent manner to both forked DNA that mimics stalled replication forks and to template/primer DNA. We found that WRNIP1 interacts physically with RAD18 and interferes with the binding of RAD18 to forked DNA and to template/primer DNA. In contrast, RAD18 enhances the binding of WRNIP1 to these DNAs, suggesting that WRNIP1 targets DNA bound by RAD18.
AB - WRN interacting protein 1 (WRNIP1) was originally identified as a protein that interacts with the Werner syndrome responsible gene product (WRN). WRNIP1 is a highly conserved protein from E. coli to humans. Genetic studies in budding yeast suggested that the yeast orthlog of WRNIP1, Mgs1, may function in a DNA damage tolerance pathway that is similar to, but distinct from, the template-switch damage avoidance pathway involving Rad6, Rad18, Rad5, Mms2, and Ubc13. Here we report that human WRNIP1 binds in an ATP dependent manner to both forked DNA that mimics stalled replication forks and to template/primer DNA. We found that WRNIP1 interacts physically with RAD18 and interferes with the binding of RAD18 to forked DNA and to template/primer DNA. In contrast, RAD18 enhances the binding of WRNIP1 to these DNAs, suggesting that WRNIP1 targets DNA bound by RAD18.
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U2 - 10.1266/ggs.84.171
DO - 10.1266/ggs.84.171
M3 - Article
VL - 84
SP - 171
EP - 178
JO - Genes and Genetic Systems
JF - Genes and Genetic Systems
SN - 1341-7568
IS - 2
ER -