Physical properties of the extracellular matrix of decellularized porcine liver

Hiroyuki Ijima, Shintaro Nakamura, Ronald Bual, Nana Shirakigawa, Shuichi Tanoue

研究成果: ジャーナルへの寄稿学術誌査読

20 被引用数 (Scopus)


The decellularization of organs has attracted attention as a new functional methodology for regenerative medicine based on tissue engineering. In previous work we developed an L-ECM (Extracellular Matrix) as a substrate-solubilized decellularized liver and demonstrated its effectiveness as a substrate for culturing and transplantation. Importantly, the physical properties of the substrate constitute important factors that control cell behavior. In this study, we aimed to quantify the physical properties of L-ECM and L-ECM gels. L-ECM was prepared as a liver-specific matrix substrate from solubilized decellularized porcine liver. In comparison to type I collagen, L-ECM yielded a lower elasticity and exhibited an abrupt decrease in its elastic modulus at 37 C. Its elastic modulus increased at increased temperatures, and the storage elastic modulus value never fell below the loss modulus value. An increase in the gel concentration of L-ECM resulted in a decrease in the biodegradation rate and in an increase in mechanical strength. The reported properties of L-ECM gel (10 mg/mL) were equivalent to those of collagen gel (3 mg/mL), which is commonly used in regenerative medicine and gel cultures. Based on reported findings, the physical properties of the novel functional substrate for culturing and regenerative medicine L-ECM were quantified.

出版ステータス出版済み - 6月 2018

!!!All Science Journal Classification (ASJC) codes

  • バイオエンジニアリング
  • 生体材料
  • 有機化学
  • ポリマーおよびプラスチック


「Physical properties of the extracellular matrix of decellularized porcine liver」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。