Physiological properties and genome structure of the hyperthermophilic filamentous phage φOH3 which infects Thermus thermophilus HB8

Yuko Nagayoshi, Kenta Kumagae, Kazuki Mori, Kosuke Tashiro, Ayano Nakamura, Yasuhiro Fujino, Yasuaki Hiromasa, Takeo Iwamoto, Satoru Kuhara, Toshihisa Ohshima, Katsumi Doi

研究成果: ジャーナルへの寄稿記事

6 引用 (Scopus)

抄録

A filamentous bacteriophage, φOH3, was isolated from hot spring sediment in Obama hot spring in Japan with the hyperthermophilic bacterium Thermus thermophilus HB8 as its host. Phage φOH3, which was classified into the Inoviridae family, consists of a flexible filamentous particle 830 nm long and 8 nm wide. φOH3 was stable at temperatures ranging from 70 to 90°C and at pHs ranging from 6 to 9. A one-step growth curve of the phage showed a 60-min latent period beginning immediately postinfection, followed by intracellular virus particle production during the subsequent 40 min. The released virion number of φOH3 was 109. During the latent period, both single stranded DNA (ssDNA) and the replicative form (RF) of phage DNA were multiplied from min 40 onward. During the release period, the copy numbers of both ssDNA and RF DNA increased sharply. The size of the φOH3 genome is 5688 bp, and eight putative open reading frames (ORFs) were annotated. These ORFs were encoded on the plus strand of RF DNA and showed no significant homology with any known phage genes, except ORF 5, which showed 60% identity with the gene VIII product of the Thermus filamentous phage PH75. All the ORFs were similar to predicted genes annotated in the Thermus aquaticus Y51MC23 and Meiothermus timidus DSM 17022 genomes at the amino acid sequence level. This is the first report of the whole genome structure and DNA multiplication of a filamentous T. thermophilus phage within its host cell.

元の言語英語
記事番号50
ジャーナルFrontiers in Microbiology
7
発行部数FEB
DOI
出版物ステータス出版済み - 2 23 2016

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Thermus thermophilus
Bacteriophages
Genome
Open Reading Frames
Thermus
Hot Springs
Single-Stranded DNA
DNA
Inoviridae
Virion
Inovirus
Genes
Genome Size
Amino Acid Sequence
Japan
Bacteria
Temperature
Growth

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

これを引用

Physiological properties and genome structure of the hyperthermophilic filamentous phage φOH3 which infects Thermus thermophilus HB8. / Nagayoshi, Yuko; Kumagae, Kenta; Mori, Kazuki; Tashiro, Kosuke; Nakamura, Ayano; Fujino, Yasuhiro; Hiromasa, Yasuaki; Iwamoto, Takeo; Kuhara, Satoru; Ohshima, Toshihisa; Doi, Katsumi.

:: Frontiers in Microbiology, 巻 7, 番号 FEB, 50, 23.02.2016.

研究成果: ジャーナルへの寄稿記事

Nagayoshi, Yuko ; Kumagae, Kenta ; Mori, Kazuki ; Tashiro, Kosuke ; Nakamura, Ayano ; Fujino, Yasuhiro ; Hiromasa, Yasuaki ; Iwamoto, Takeo ; Kuhara, Satoru ; Ohshima, Toshihisa ; Doi, Katsumi. / Physiological properties and genome structure of the hyperthermophilic filamentous phage φOH3 which infects Thermus thermophilus HB8. :: Frontiers in Microbiology. 2016 ; 巻 7, 番号 FEB.
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AU - Nagayoshi, Yuko

AU - Kumagae, Kenta

AU - Mori, Kazuki

AU - Tashiro, Kosuke

AU - Nakamura, Ayano

AU - Fujino, Yasuhiro

AU - Hiromasa, Yasuaki

AU - Iwamoto, Takeo

AU - Kuhara, Satoru

AU - Ohshima, Toshihisa

AU - Doi, Katsumi

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AB - A filamentous bacteriophage, φOH3, was isolated from hot spring sediment in Obama hot spring in Japan with the hyperthermophilic bacterium Thermus thermophilus HB8 as its host. Phage φOH3, which was classified into the Inoviridae family, consists of a flexible filamentous particle 830 nm long and 8 nm wide. φOH3 was stable at temperatures ranging from 70 to 90°C and at pHs ranging from 6 to 9. A one-step growth curve of the phage showed a 60-min latent period beginning immediately postinfection, followed by intracellular virus particle production during the subsequent 40 min. The released virion number of φOH3 was 109. During the latent period, both single stranded DNA (ssDNA) and the replicative form (RF) of phage DNA were multiplied from min 40 onward. During the release period, the copy numbers of both ssDNA and RF DNA increased sharply. The size of the φOH3 genome is 5688 bp, and eight putative open reading frames (ORFs) were annotated. These ORFs were encoded on the plus strand of RF DNA and showed no significant homology with any known phage genes, except ORF 5, which showed 60% identity with the gene VIII product of the Thermus filamentous phage PH75. All the ORFs were similar to predicted genes annotated in the Thermus aquaticus Y51MC23 and Meiothermus timidus DSM 17022 genomes at the amino acid sequence level. This is the first report of the whole genome structure and DNA multiplication of a filamentous T. thermophilus phage within its host cell.

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