Phytostilbenoid production in white mulberry (Morus alba L.) cell culture using bioreactors and simple deglycosylation by endogenous enzymatic hydrolysis

Jukrapun Komaikul, Tharita Kitisripanya, Chadathorn Inyai, Kittisak Likhitwitayawuid, Boonchoo Sritularak, Hiroyuki Tanaka, Waraporn Putalun

研究成果: ジャーナルへの寄稿記事

抄録

Phytostilbenes are responsible for several biological activities of mulberry (Morus sp.), which has been widely used as a raw material in health products. This study aimed to investigate the capability of Morus alba L. cell in bioreactors to produce the major bioactive stilbenes. The cell obtained from air-driven bioreactors such as round bottom, flat bottom, and air-lift vessel shape bioreactors was collected and analyzed for the levels of mulberroside A and oxyresveratrol. The results showed that the cell culture in round bottom and air-lift vessel bioreactors had higher growth rate, as compared with the cell culture in shake flasks (1.38- and 1.41-fold, respectively). The optimized culture condition to produce mulberroside A was obtained from round bottom bioreactor culture (55.56 ± 11.41 μmol/L). Additionally, endogenous stilbenoid hydrolysis of cell from the bioreactor culture was examined. Under optimized hydrolytic conditions, mulberroside A in the cell was readily deglycosylated to give oxyresveratrol within 1 h. These results indicated that the glycoside mulberroside A in the cell is sensitive to the endogenous enzymatic hydrolysis. Interaction of the stilbenoid components with the endogenous hydrolytic enzyme triggered by cell disruption in M. alba samples was suggested to be the major cause of the alteration of the stilbenoid levels. These findings have provided a new approach to producing glycosidic compounds and corresponding aglycones in cell culture.

元の言語英語
ページ(範囲)199-208
ページ数10
ジャーナルIn Vitro Cellular and Developmental Biology - Plant
55
発行部数2
DOI
出版物ステータス出版済み - 4 15 2019

Fingerprint

Morus
Morus alba
Bioreactors
enzymatic hydrolysis
bioreactors
cell culture
Hydrolysis
Cell Culture Techniques
stilbenoids
Air
cells
air
Stilbenes
Morus (Moraceae)
stilbenes
mulberries
Glycosides
glycosides
raw materials
bioactive properties

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Plant Science

これを引用

Phytostilbenoid production in white mulberry (Morus alba L.) cell culture using bioreactors and simple deglycosylation by endogenous enzymatic hydrolysis. / Komaikul, Jukrapun; Kitisripanya, Tharita; Inyai, Chadathorn; Likhitwitayawuid, Kittisak; Sritularak, Boonchoo; Tanaka, Hiroyuki; Putalun, Waraporn.

:: In Vitro Cellular and Developmental Biology - Plant, 巻 55, 番号 2, 15.04.2019, p. 199-208.

研究成果: ジャーナルへの寄稿記事

Komaikul, Jukrapun ; Kitisripanya, Tharita ; Inyai, Chadathorn ; Likhitwitayawuid, Kittisak ; Sritularak, Boonchoo ; Tanaka, Hiroyuki ; Putalun, Waraporn. / Phytostilbenoid production in white mulberry (Morus alba L.) cell culture using bioreactors and simple deglycosylation by endogenous enzymatic hydrolysis. :: In Vitro Cellular and Developmental Biology - Plant. 2019 ; 巻 55, 番号 2. pp. 199-208.
@article{93b6675b4a2b465f9a7f80755fe61948,
title = "Phytostilbenoid production in white mulberry (Morus alba L.) cell culture using bioreactors and simple deglycosylation by endogenous enzymatic hydrolysis",
abstract = "Phytostilbenes are responsible for several biological activities of mulberry (Morus sp.), which has been widely used as a raw material in health products. This study aimed to investigate the capability of Morus alba L. cell in bioreactors to produce the major bioactive stilbenes. The cell obtained from air-driven bioreactors such as round bottom, flat bottom, and air-lift vessel shape bioreactors was collected and analyzed for the levels of mulberroside A and oxyresveratrol. The results showed that the cell culture in round bottom and air-lift vessel bioreactors had higher growth rate, as compared with the cell culture in shake flasks (1.38- and 1.41-fold, respectively). The optimized culture condition to produce mulberroside A was obtained from round bottom bioreactor culture (55.56 ± 11.41 μmol/L). Additionally, endogenous stilbenoid hydrolysis of cell from the bioreactor culture was examined. Under optimized hydrolytic conditions, mulberroside A in the cell was readily deglycosylated to give oxyresveratrol within 1 h. These results indicated that the glycoside mulberroside A in the cell is sensitive to the endogenous enzymatic hydrolysis. Interaction of the stilbenoid components with the endogenous hydrolytic enzyme triggered by cell disruption in M. alba samples was suggested to be the major cause of the alteration of the stilbenoid levels. These findings have provided a new approach to producing glycosidic compounds and corresponding aglycones in cell culture.",
author = "Jukrapun Komaikul and Tharita Kitisripanya and Chadathorn Inyai and Kittisak Likhitwitayawuid and Boonchoo Sritularak and Hiroyuki Tanaka and Waraporn Putalun",
year = "2019",
month = "4",
day = "15",
doi = "10.1007/s11627-018-09953-3",
language = "English",
volume = "55",
pages = "199--208",
journal = "In Vitro Cellular and Developmental Biology - Plant",
issn = "1054-5476",
publisher = "Springer New York",
number = "2",

}

TY - JOUR

T1 - Phytostilbenoid production in white mulberry (Morus alba L.) cell culture using bioreactors and simple deglycosylation by endogenous enzymatic hydrolysis

AU - Komaikul, Jukrapun

AU - Kitisripanya, Tharita

AU - Inyai, Chadathorn

AU - Likhitwitayawuid, Kittisak

AU - Sritularak, Boonchoo

AU - Tanaka, Hiroyuki

AU - Putalun, Waraporn

PY - 2019/4/15

Y1 - 2019/4/15

N2 - Phytostilbenes are responsible for several biological activities of mulberry (Morus sp.), which has been widely used as a raw material in health products. This study aimed to investigate the capability of Morus alba L. cell in bioreactors to produce the major bioactive stilbenes. The cell obtained from air-driven bioreactors such as round bottom, flat bottom, and air-lift vessel shape bioreactors was collected and analyzed for the levels of mulberroside A and oxyresveratrol. The results showed that the cell culture in round bottom and air-lift vessel bioreactors had higher growth rate, as compared with the cell culture in shake flasks (1.38- and 1.41-fold, respectively). The optimized culture condition to produce mulberroside A was obtained from round bottom bioreactor culture (55.56 ± 11.41 μmol/L). Additionally, endogenous stilbenoid hydrolysis of cell from the bioreactor culture was examined. Under optimized hydrolytic conditions, mulberroside A in the cell was readily deglycosylated to give oxyresveratrol within 1 h. These results indicated that the glycoside mulberroside A in the cell is sensitive to the endogenous enzymatic hydrolysis. Interaction of the stilbenoid components with the endogenous hydrolytic enzyme triggered by cell disruption in M. alba samples was suggested to be the major cause of the alteration of the stilbenoid levels. These findings have provided a new approach to producing glycosidic compounds and corresponding aglycones in cell culture.

AB - Phytostilbenes are responsible for several biological activities of mulberry (Morus sp.), which has been widely used as a raw material in health products. This study aimed to investigate the capability of Morus alba L. cell in bioreactors to produce the major bioactive stilbenes. The cell obtained from air-driven bioreactors such as round bottom, flat bottom, and air-lift vessel shape bioreactors was collected and analyzed for the levels of mulberroside A and oxyresveratrol. The results showed that the cell culture in round bottom and air-lift vessel bioreactors had higher growth rate, as compared with the cell culture in shake flasks (1.38- and 1.41-fold, respectively). The optimized culture condition to produce mulberroside A was obtained from round bottom bioreactor culture (55.56 ± 11.41 μmol/L). Additionally, endogenous stilbenoid hydrolysis of cell from the bioreactor culture was examined. Under optimized hydrolytic conditions, mulberroside A in the cell was readily deglycosylated to give oxyresveratrol within 1 h. These results indicated that the glycoside mulberroside A in the cell is sensitive to the endogenous enzymatic hydrolysis. Interaction of the stilbenoid components with the endogenous hydrolytic enzyme triggered by cell disruption in M. alba samples was suggested to be the major cause of the alteration of the stilbenoid levels. These findings have provided a new approach to producing glycosidic compounds and corresponding aglycones in cell culture.

UR - http://www.scopus.com/inward/record.url?scp=85062817354&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85062817354&partnerID=8YFLogxK

U2 - 10.1007/s11627-018-09953-3

DO - 10.1007/s11627-018-09953-3

M3 - Article

AN - SCOPUS:85062817354

VL - 55

SP - 199

EP - 208

JO - In Vitro Cellular and Developmental Biology - Plant

JF - In Vitro Cellular and Developmental Biology - Plant

SN - 1054-5476

IS - 2

ER -