Piezo Electric Sensor for Endocrine-Disrupting Chemicals Using Receptor-co-factor Interaction

Masaharu Murata, Chifumi Gouda, Kentaro Yano, Shinichiro Kuroki, Tatsuo Suzutani, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

8 引用 (Scopus)

抄録

In vitro screening assays are useful techniques for the determination of receptor-mediated activities in environmental samples. In order to define whether environmental chemicals act as an agonist or antagonist to the human estrogen receptor (hER), we have constructed a biosensor based on ligand-inducible interactions between hER and relative proteins on a quartz crystal microbalance (QCM). The his-tagged proteins, which were expressed in E. coli by recombinant DNA technology, were immobilized on an Au-electrode with Ni(II)-mediated chemisorption using the histidine tag and thiol-modified iminodiacetic acid. The resonance-frequency change of the protein-modified electrode was caused by association or dissociation with the hER relative proteins on the surface in the presence of estrogen. These results suggest that this sensor is applicable as a large-scale screening tool for estrogenic compounds.

元の言語英語
ページ(範囲)1355-1357
ページ数3
ジャーナルanalytical sciences
19
発行部数10
DOI
出版物ステータス出版済み - 1 1 2003

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Endocrine Disruptors
Estrogen Receptors
Sensors
Screening
Estrogens
Proteins
Genetic engineering
Electrodes
Quartz crystal microbalances
Chemisorption
Histidine
Sulfhydryl Compounds
Biosensors
Escherichia coli
Assays
Association reactions
Ligands

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

これを引用

Piezo Electric Sensor for Endocrine-Disrupting Chemicals Using Receptor-co-factor Interaction. / Murata, Masaharu; Gouda, Chifumi; Yano, Kentaro; Kuroki, Shinichiro; Suzutani, Tatsuo; Katayama, Yoshiki.

:: analytical sciences, 巻 19, 番号 10, 01.01.2003, p. 1355-1357.

研究成果: ジャーナルへの寄稿記事

Murata, Masaharu ; Gouda, Chifumi ; Yano, Kentaro ; Kuroki, Shinichiro ; Suzutani, Tatsuo ; Katayama, Yoshiki. / Piezo Electric Sensor for Endocrine-Disrupting Chemicals Using Receptor-co-factor Interaction. :: analytical sciences. 2003 ; 巻 19, 番号 10. pp. 1355-1357.
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