Plasmid vectors designed for high-efficiency expression controlled by the portable recA promoteroperator of Escherichia coli

Shirakawa Masahiro, Tsurimoto Toshiki, Matsubara Kenichi

研究成果: Contribution to journalArticle査読

32 被引用数 (Scopus)

抄録

A novel expression vector using the 236-bp promoter-operator fragment of the recA gene (recApo) of Escherichia coli has been constructed. This DNA fragment contains complete signals for the initiation of RNA synthesis, as well as for regulation by the lexA product, but lacks the coding sequence for the RecA protein. The strength of the recA promoter was examined by assaying ß-galactosidase activity expressed from a cro-lacZ fused gene placed downstream of the promoter. Under noninducing conditions, the promoter was regulated by the LexA protein, and the fused gene was expressed only weakly. Upon induction by nalidixic acid in a recA+ strain, high expression was observed for an extended period. After 5 h under inducing conditions, as much as 11 % of the total cellular protein was cro-lacZ product. The expression level was higher than that from promoters of lac, trp, and λ early genes.

本文言語英語
ページ(範囲)127-132
ページ数6
ジャーナルGene
28
1
DOI
出版ステータス出版済み - 4 1984

All Science Journal Classification (ASJC) codes

  • Genetics

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