Plate assay for endo-β-N-acetylglucosaminidase activity using a chromogenic substrate synthesized by transglycosylation with Arthrobacter endo-β-N-acetylglucosaminidase

Kiyotaka Fujita; Yasuhiko Asada; Kenji Yamamoto; Kaoru Takegawa

doi:10.1016/S1389-1723(01)80021-9

Plate assay for endo-β-N-acetylglucosaminidase activity using a chromogenic substrate synthesized by transglycosylation with Arthrobacter endo-β-N-acetylglucosaminidase

Kiyotaka Fujita, Yasuhiko Asada, Kenji Yamamoto, Kaoru Takegawa

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

抄録

The transglycosylation activity of Arthrobacter endo-β-N-acetylglucosaminidase (Endo-A) was used for the enzymatic synthesis of a novel oligosaccharide, Man₆GlcNAc-5-bromo-4-chloro-3-indolyl-β-glucoside (Man₆GlcNAc-Glc-β-X). Various endo-β-N-acetylglucosaminidases hydrolyzed this oligosaccharide, producing Man₆GlcNAc and Glc-β-X. The E. coli strains coexpressing Endo-A and β-glucosidase formed blue colonies in the presence of Man₆GlcNAc-Glc-β-X. Therefore, endo-β-N-acetylglucosaminidase activity could be directly detected by the plate assay. This simple plate assay is useful for screening microorganisms for endo-β-N-acetylglucosaminidase activity.

本文言語	英語
ページ（範囲）	462-464
ページ数	3
ジャーナル	Journal of Bioscience and Bioengineering
巻	90
号	4
DOI	https://doi.org/10.1016/S1389-1723(01)80021-9
出版ステータス	出版済み - 10月 2000
外部発表	はい

!!!All Science Journal Classification (ASJC) codes

バイオテクノロジー
バイオエンジニアリング
応用微生物学とバイオテクノロジー

文献へのアクセス

10.1016/S1389-1723(01)80021-9

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引用スタイル

Plate assay for endo-β-N-acetylglucosaminidase activity using a chromogenic substrate synthesized by transglycosylation with Arthrobacter endo-β-N-acetylglucosaminidase. / Fujita, Kiyotaka; Asada, Yasuhiko; Yamamoto, Kenji その他.
In: Journal of Bioscience and Bioengineering, Vol. 90, No. 4, 10.2000, p. 462-464.

研究成果: ジャーナルへの寄稿 › 学術誌 › 査読

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abstract = "The transglycosylation activity of Arthrobacter endo-β-N-acetylglucosaminidase (Endo-A) was used for the enzymatic synthesis of a novel oligosaccharide, Man6GlcNAc-5-bromo-4-chloro-3-indolyl-β-glucoside (Man6GlcNAc-Glc-β-X). Various endo-β-N-acetylglucosaminidases hydrolyzed this oligosaccharide, producing Man6GlcNAc and Glc-β-X. The E. coli strains coexpressing Endo-A and β-glucosidase formed blue colonies in the presence of Man6GlcNAc-Glc-β-X. Therefore, endo-β-N-acetylglucosaminidase activity could be directly detected by the plate assay. This simple plate assay is useful for screening microorganisms for endo-β-N-acetylglucosaminidase activity.",

author = "Kiyotaka Fujita and Yasuhiko Asada and Kenji Yamamoto and Kaoru Takegawa",

note = "Funding Information: We are grateful to Drs. Mutsumi Sano and Akihiro Kondo (Takara Shuzo Co.) for providing E. coli plasmids and many helpful discussions during the course of this study. This work was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan (to K.T.).",

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