Plk1 phosphorylates CLIP-170 and regulates its binding to microtubules for chromosome alignment

Mai Kakeno, Kenji Matsuzawa, Toshinori Matsui, Hiroki Akita, Ikuko Sugiyama, Fumiyoshi Ishidate, Atsushi Nakano, Seiji Takashima, Hidemasa Goto, Masaki Inagaki, Kozo Kaibuchi, Takashi Watanabe

研究成果: ジャーナルへの寄稿記事

9 引用 (Scopus)

抄録

The microtubule (MT) cytoskeleton is essential for cellular morphogenesis, cell migration, and cell division. MT organization is primarily mediated by a variety of MT-associated proteins. Among these proteins, plus-end-tracking proteins (+TIPs) are evolutionarily conserved factors that selectively accumulate at growing MT plus ends. Cytoplasmic linker protein (CLIP)-170 is a +TIP that associates with diverse proteins to determine the behavior of MT ends and their linkage to intracellular structures, including mitotic chromosomes. However, how CLIP-170 activity is spatially and temporally controlled is largely unknown. Here, we show that phosphorylation at Ser312 in the third serine-rich region of CLIP-170 is increased during mitosis. Polo-like kinase 1 (Plk1) is responsible for this phosphorylation during the mitotic phase of dividing cells. In vitro analysis using a purified CLIP-170 N-terminal fragment showed that phosphorylation by Plk1 diminishes CLIP-170 binding to the MT ends and lattice without affecting binding to EB3. Furthermore, we demonstrate that during mitosis, stable kinetochore/MT attachment and subsequent chromosome alignment require CLIP-170 and a proper phosphorylation/dephosphorylation cycle at Ser312. We propose that CLIP-170 phosphorylation by Plk1 regulates proper chromosome alignment by modulating the interaction between CLIP-170 and MTs in mitotic cells and that CLIP-170 activity is stringently controlled by its phosphorylation state, which depends on the cellular context.

元の言語英語
ページ(範囲)45-59
ページ数15
ジャーナルCell structure and function
39
発行部数1
DOI
出版物ステータス出版済み - 1 1 2014
外部発表Yes

Fingerprint

Microtubules
Chromosomes
Phosphorylation
Mitosis
polo-like kinase 1
cytoplasmic linker protein 170
Kinetochores
Proteins
Microtubule-Associated Proteins
Cytoskeleton
Morphogenesis
Protein Binding
Cell Division
Serine
Cell Movement

All Science Journal Classification (ASJC) codes

  • Physiology
  • Molecular Biology
  • Cell Biology

これを引用

Plk1 phosphorylates CLIP-170 and regulates its binding to microtubules for chromosome alignment. / Kakeno, Mai; Matsuzawa, Kenji; Matsui, Toshinori; Akita, Hiroki; Sugiyama, Ikuko; Ishidate, Fumiyoshi; Nakano, Atsushi; Takashima, Seiji; Goto, Hidemasa; Inagaki, Masaki; Kaibuchi, Kozo; Watanabe, Takashi.

:: Cell structure and function, 巻 39, 番号 1, 01.01.2014, p. 45-59.

研究成果: ジャーナルへの寄稿記事

Kakeno, M, Matsuzawa, K, Matsui, T, Akita, H, Sugiyama, I, Ishidate, F, Nakano, A, Takashima, S, Goto, H, Inagaki, M, Kaibuchi, K & Watanabe, T 2014, 'Plk1 phosphorylates CLIP-170 and regulates its binding to microtubules for chromosome alignment', Cell structure and function, 巻. 39, 番号 1, pp. 45-59. https://doi.org/10.1247/csf.14001
Kakeno, Mai ; Matsuzawa, Kenji ; Matsui, Toshinori ; Akita, Hiroki ; Sugiyama, Ikuko ; Ishidate, Fumiyoshi ; Nakano, Atsushi ; Takashima, Seiji ; Goto, Hidemasa ; Inagaki, Masaki ; Kaibuchi, Kozo ; Watanabe, Takashi. / Plk1 phosphorylates CLIP-170 and regulates its binding to microtubules for chromosome alignment. :: Cell structure and function. 2014 ; 巻 39, 番号 1. pp. 45-59.
@article{c1a7f418d3654b72b5e1b8a8da9c4a40,
title = "Plk1 phosphorylates CLIP-170 and regulates its binding to microtubules for chromosome alignment",
abstract = "The microtubule (MT) cytoskeleton is essential for cellular morphogenesis, cell migration, and cell division. MT organization is primarily mediated by a variety of MT-associated proteins. Among these proteins, plus-end-tracking proteins (+TIPs) are evolutionarily conserved factors that selectively accumulate at growing MT plus ends. Cytoplasmic linker protein (CLIP)-170 is a +TIP that associates with diverse proteins to determine the behavior of MT ends and their linkage to intracellular structures, including mitotic chromosomes. However, how CLIP-170 activity is spatially and temporally controlled is largely unknown. Here, we show that phosphorylation at Ser312 in the third serine-rich region of CLIP-170 is increased during mitosis. Polo-like kinase 1 (Plk1) is responsible for this phosphorylation during the mitotic phase of dividing cells. In vitro analysis using a purified CLIP-170 N-terminal fragment showed that phosphorylation by Plk1 diminishes CLIP-170 binding to the MT ends and lattice without affecting binding to EB3. Furthermore, we demonstrate that during mitosis, stable kinetochore/MT attachment and subsequent chromosome alignment require CLIP-170 and a proper phosphorylation/dephosphorylation cycle at Ser312. We propose that CLIP-170 phosphorylation by Plk1 regulates proper chromosome alignment by modulating the interaction between CLIP-170 and MTs in mitotic cells and that CLIP-170 activity is stringently controlled by its phosphorylation state, which depends on the cellular context.",
author = "Mai Kakeno and Kenji Matsuzawa and Toshinori Matsui and Hiroki Akita and Ikuko Sugiyama and Fumiyoshi Ishidate and Atsushi Nakano and Seiji Takashima and Hidemasa Goto and Masaki Inagaki and Kozo Kaibuchi and Takashi Watanabe",
year = "2014",
month = "1",
day = "1",
doi = "10.1247/csf.14001",
language = "English",
volume = "39",
pages = "45--59",
journal = "Cell Structure and Function",
issn = "0386-7196",
publisher = "Japan Society for Cell Biology",
number = "1",

}

TY - JOUR

T1 - Plk1 phosphorylates CLIP-170 and regulates its binding to microtubules for chromosome alignment

AU - Kakeno, Mai

AU - Matsuzawa, Kenji

AU - Matsui, Toshinori

AU - Akita, Hiroki

AU - Sugiyama, Ikuko

AU - Ishidate, Fumiyoshi

AU - Nakano, Atsushi

AU - Takashima, Seiji

AU - Goto, Hidemasa

AU - Inagaki, Masaki

AU - Kaibuchi, Kozo

AU - Watanabe, Takashi

PY - 2014/1/1

Y1 - 2014/1/1

N2 - The microtubule (MT) cytoskeleton is essential for cellular morphogenesis, cell migration, and cell division. MT organization is primarily mediated by a variety of MT-associated proteins. Among these proteins, plus-end-tracking proteins (+TIPs) are evolutionarily conserved factors that selectively accumulate at growing MT plus ends. Cytoplasmic linker protein (CLIP)-170 is a +TIP that associates with diverse proteins to determine the behavior of MT ends and their linkage to intracellular structures, including mitotic chromosomes. However, how CLIP-170 activity is spatially and temporally controlled is largely unknown. Here, we show that phosphorylation at Ser312 in the third serine-rich region of CLIP-170 is increased during mitosis. Polo-like kinase 1 (Plk1) is responsible for this phosphorylation during the mitotic phase of dividing cells. In vitro analysis using a purified CLIP-170 N-terminal fragment showed that phosphorylation by Plk1 diminishes CLIP-170 binding to the MT ends and lattice without affecting binding to EB3. Furthermore, we demonstrate that during mitosis, stable kinetochore/MT attachment and subsequent chromosome alignment require CLIP-170 and a proper phosphorylation/dephosphorylation cycle at Ser312. We propose that CLIP-170 phosphorylation by Plk1 regulates proper chromosome alignment by modulating the interaction between CLIP-170 and MTs in mitotic cells and that CLIP-170 activity is stringently controlled by its phosphorylation state, which depends on the cellular context.

AB - The microtubule (MT) cytoskeleton is essential for cellular morphogenesis, cell migration, and cell division. MT organization is primarily mediated by a variety of MT-associated proteins. Among these proteins, plus-end-tracking proteins (+TIPs) are evolutionarily conserved factors that selectively accumulate at growing MT plus ends. Cytoplasmic linker protein (CLIP)-170 is a +TIP that associates with diverse proteins to determine the behavior of MT ends and their linkage to intracellular structures, including mitotic chromosomes. However, how CLIP-170 activity is spatially and temporally controlled is largely unknown. Here, we show that phosphorylation at Ser312 in the third serine-rich region of CLIP-170 is increased during mitosis. Polo-like kinase 1 (Plk1) is responsible for this phosphorylation during the mitotic phase of dividing cells. In vitro analysis using a purified CLIP-170 N-terminal fragment showed that phosphorylation by Plk1 diminishes CLIP-170 binding to the MT ends and lattice without affecting binding to EB3. Furthermore, we demonstrate that during mitosis, stable kinetochore/MT attachment and subsequent chromosome alignment require CLIP-170 and a proper phosphorylation/dephosphorylation cycle at Ser312. We propose that CLIP-170 phosphorylation by Plk1 regulates proper chromosome alignment by modulating the interaction between CLIP-170 and MTs in mitotic cells and that CLIP-170 activity is stringently controlled by its phosphorylation state, which depends on the cellular context.

UR - http://www.scopus.com/inward/record.url?scp=84896869967&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84896869967&partnerID=8YFLogxK

U2 - 10.1247/csf.14001

DO - 10.1247/csf.14001

M3 - Article

C2 - 24451569

AN - SCOPUS:84896869967

VL - 39

SP - 45

EP - 59

JO - Cell Structure and Function

JF - Cell Structure and Function

SN - 0386-7196

IS - 1

ER -