PNA-tagged peptide microarrays for ratiometric activity detection of cellular protein kinases

Hiromu Ikeda, Yoshihiro Yayama, Akito Hata, Jumpei Kamimoto, Tatsuhiro Yamamoto, Takeshi Mori, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

抄録

Peptide microarrays can be used to measure the activity of multiple protein kinases (PKs), which can be used to elucidate kinomics for drug discovery and diagnosis. Here we demonstrated a new microarray for ratiometric detection of the activity of PKs using peptide nucleic acid (PNA)-tagged peptides labeled with two different fluorophores, Cy3 and Cy5. We successfully detected cellular PK activities based on ratiometry, and applied the system for evaluation of an inhibitory drug.

元の言語英語
ページ(範囲)631-635
ページ数5
ジャーナルanalytical sciences
30
発行部数6
DOI
出版物ステータス出版済み - 1 1 2014

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Peptide Nucleic Acids
Microarrays
Protein Kinases
Peptides
Fluorophores
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

これを引用

PNA-tagged peptide microarrays for ratiometric activity detection of cellular protein kinases. / Ikeda, Hiromu; Yayama, Yoshihiro; Hata, Akito; Kamimoto, Jumpei; Yamamoto, Tatsuhiro; Mori, Takeshi; Katayama, Yoshiki.

:: analytical sciences, 巻 30, 番号 6, 01.01.2014, p. 631-635.

研究成果: ジャーナルへの寄稿記事

Ikeda, Hiromu ; Yayama, Yoshihiro ; Hata, Akito ; Kamimoto, Jumpei ; Yamamoto, Tatsuhiro ; Mori, Takeshi ; Katayama, Yoshiki. / PNA-tagged peptide microarrays for ratiometric activity detection of cellular protein kinases. :: analytical sciences. 2014 ; 巻 30, 番号 6. pp. 631-635.
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