Polo-like kinase Cdc5 regulates Spc72 recruitment to spindle pole body in the methylotrophic yeast ogataea polymorpha

Hiromi Maekawa, Annett Neuner, Diana Rüthnick, Elmar Schiebel, Gislene Pereira, Yoshinobu Kaneko

研究成果: ジャーナルへの寄稿記事

抄録

Cytoplasmic microtubules (cMT) control mitotic spindle positioning in many organisms, and are therefore pivotal for successful cell division. Despite its importance, the temporal control of cMT formation remains poorly understood. Here we show that unlike the best-studied yeast Saccharomyces cerevisiae, position of pre-anaphase nucleus is not strongly biased toward bud neck in Ogataea polymorpha and the regulation of spindle positioning becomes active only shortly before anaphase. This is likely due to the unstable property of cMTs compared to those in S. cerevisiae. Furthermore, we show that cMT nucleation/anchoring is restricted at the level of recruitment of the γ-tubulin complex receptor, Spc72, to spindle pole body (SPB), which is regulated by the polo-like kinase Cdc5. Additionally, electron microscopy revealed that the cytoplasmic side of SPB is structurally different between G1 and anaphase. Thus, polo-like kinase dependent recruitment of γ-tubulin receptor to SPBs determines the timing of spindle orientation in O. polymorpha.

元の言語英語
記事番号e24340
ジャーナルeLife
6
DOI
出版物ステータス出版済み - 8 30 2017

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Spindle Pole Bodies
Anaphase
Microtubules
Yeast
Poles
Phosphotransferases
Yeasts
Tubulin
Saccharomyces cerevisiae
Spindle Apparatus
Cell Division
Electron microscopy
Electron Microscopy
Nucleation
Cells

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

これを引用

Polo-like kinase Cdc5 regulates Spc72 recruitment to spindle pole body in the methylotrophic yeast ogataea polymorpha. / Maekawa, Hiromi; Neuner, Annett; Rüthnick, Diana; Schiebel, Elmar; Pereira, Gislene; Kaneko, Yoshinobu.

:: eLife, 巻 6, e24340, 30.08.2017.

研究成果: ジャーナルへの寄稿記事

Maekawa, Hiromi ; Neuner, Annett ; Rüthnick, Diana ; Schiebel, Elmar ; Pereira, Gislene ; Kaneko, Yoshinobu. / Polo-like kinase Cdc5 regulates Spc72 recruitment to spindle pole body in the methylotrophic yeast ogataea polymorpha. :: eLife. 2017 ; 巻 6.
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abstract = "Cytoplasmic microtubules (cMT) control mitotic spindle positioning in many organisms, and are therefore pivotal for successful cell division. Despite its importance, the temporal control of cMT formation remains poorly understood. Here we show that unlike the best-studied yeast Saccharomyces cerevisiae, position of pre-anaphase nucleus is not strongly biased toward bud neck in Ogataea polymorpha and the regulation of spindle positioning becomes active only shortly before anaphase. This is likely due to the unstable property of cMTs compared to those in S. cerevisiae. Furthermore, we show that cMT nucleation/anchoring is restricted at the level of recruitment of the γ-tubulin complex receptor, Spc72, to spindle pole body (SPB), which is regulated by the polo-like kinase Cdc5. Additionally, electron microscopy revealed that the cytoplasmic side of SPB is structurally different between G1 and anaphase. Thus, polo-like kinase dependent recruitment of γ-tubulin receptor to SPBs determines the timing of spindle orientation in O. polymorpha.",
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AU - Maekawa, Hiromi

AU - Neuner, Annett

AU - Rüthnick, Diana

AU - Schiebel, Elmar

AU - Pereira, Gislene

AU - Kaneko, Yoshinobu

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