Poly (methyl methacrylate) microchip gel electrophoresis for the concentration and separation of aptamer and aptamer-thrombin complex with discontinuous buffers

Jun Wang, Yong Zhang, Yeon Su Park, Yukihiro Okamoto, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

研究成果: Chapter in Book/Report/Conference proceedingConference contribution

抄録

A simple continuous isotachophoresis (ITP)-based microchip gel electrophoresis (MGE) was firstly applied for the concentration and separation of aptamer and its thrombin complex in a single cross-form poly (methyl methacrylate) (PMMA) microchip. The aptamer and its thrombin complex can be simultaneously concentrated and separated by this method which does not require time-consuming steps and complicated chip design. We found that different terminating electrolyte (TE) ions lead to different concentration enhancement factors and that lower mobility of TE ions can get higher concentration results. After optimization of various impact factors, we successfully achieved about 2000-fold concentration of aptamer-thrombin complex.

本文言語英語
ホスト出版物のタイトルProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
出版社Chemical and Biological Microsystems Society
ページ791-793
ページ数3
ISBN(印刷版)9780979806421
出版ステータス出版済み - 1 1 2009
外部発表はい
イベント13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, 大韓民国
継続期間: 11 1 200911 5 2009

出版物シリーズ

名前Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

その他

その他13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
国/地域大韓民国
CityJeju
Period11/1/0911/5/09

All Science Journal Classification (ASJC) codes

  • 化学工学(その他)
  • バイオエンジニアリング

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