Polyamine flux suppresses histone lysine demethylases and enhances ID1 expression in cancer stem cells

Keisuke Tamari, Masamitsu Konno, Ayumu Asai, Jun Koseki, Kazuhiko Hayashi, Koichi Kawamoto, Noriyuki Murai, Senya Matsufuji, Fumiaki Isohashi, Taroh Satoh, Noriko Goto, Shinji Tanaka, Yuichiro Doki, Masaki Mori, Kazuhiko Ogawa, Hideshi Ishii

研究成果: ジャーナルへの寄稿記事

1 引用 (Scopus)

抄録

Cancer stem cells (CSCs) exhibit tumorigenic potential and can generate resistance to chemotherapy and radiotherapy. A labeled ornithine decarboxylase (ODC, a rate-limiting enzyme involved in polyamine [PA] biosynthesis) degradation motif (degron) system allows visualization of a fraction of CSC-like cells in heterogeneous tumor populations. A labeled ODC degradation motif system allowed visualization of a fraction of CSC-like cells in heterogeneous tumor populations. Using this system, analysis of polyamine flux indicated that polyamine metabolism is active in CSCs. The results showed that intracellular polyamines inhibited the activity of histone lysine 4 demethylase enzymes, including lysine-specific demethylase-1 (LSD1). Chromatin immunoprecipitation with Pol II antibody followed by massively parallel DNA sequencing, revealed the global enrichment of Pol II in transcription start sites in CSCs. Increase of polyamines within cells resulted in an enhancement of ID1 gene expression. The results of this study reveal details of metabolic pathways that drive epigenetic control of cancer cell stemness and determine effective therapeutic targets in CSCs.

元の言語英語
記事番号104
ジャーナルCell Death Discovery
4
発行部数1
DOI
出版物ステータス出版済み - 12 1 2018

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Histone Demethylases
Neoplastic Stem Cells
Polyamines
High-Throughput Nucleotide Sequencing
Neoplasms
Ornithine Decarboxylase
Chromatin Immunoprecipitation
Transcription Initiation Site
Enzymes
Systems Analysis
Metabolic Networks and Pathways
DNA Sequence Analysis
Epigenomics
Population
Lysine
Radiotherapy
Gene Expression
Drug Therapy
Antibodies

All Science Journal Classification (ASJC) codes

  • Immunology
  • Cellular and Molecular Neuroscience
  • Cell Biology
  • Cancer Research

これを引用

Tamari, K., Konno, M., Asai, A., Koseki, J., Hayashi, K., Kawamoto, K., ... Ishii, H. (2018). Polyamine flux suppresses histone lysine demethylases and enhances ID1 expression in cancer stem cells. Cell Death Discovery, 4(1), [104]. https://doi.org/10.1038/s41420-018-0117-7

Polyamine flux suppresses histone lysine demethylases and enhances ID1 expression in cancer stem cells. / Tamari, Keisuke; Konno, Masamitsu; Asai, Ayumu; Koseki, Jun; Hayashi, Kazuhiko; Kawamoto, Koichi; Murai, Noriyuki; Matsufuji, Senya; Isohashi, Fumiaki; Satoh, Taroh; Goto, Noriko; Tanaka, Shinji; Doki, Yuichiro; Mori, Masaki; Ogawa, Kazuhiko; Ishii, Hideshi.

:: Cell Death Discovery, 巻 4, 番号 1, 104, 01.12.2018.

研究成果: ジャーナルへの寄稿記事

Tamari, K, Konno, M, Asai, A, Koseki, J, Hayashi, K, Kawamoto, K, Murai, N, Matsufuji, S, Isohashi, F, Satoh, T, Goto, N, Tanaka, S, Doki, Y, Mori, M, Ogawa, K & Ishii, H 2018, 'Polyamine flux suppresses histone lysine demethylases and enhances ID1 expression in cancer stem cells', Cell Death Discovery, 巻. 4, 番号 1, 104. https://doi.org/10.1038/s41420-018-0117-7
Tamari, Keisuke ; Konno, Masamitsu ; Asai, Ayumu ; Koseki, Jun ; Hayashi, Kazuhiko ; Kawamoto, Koichi ; Murai, Noriyuki ; Matsufuji, Senya ; Isohashi, Fumiaki ; Satoh, Taroh ; Goto, Noriko ; Tanaka, Shinji ; Doki, Yuichiro ; Mori, Masaki ; Ogawa, Kazuhiko ; Ishii, Hideshi. / Polyamine flux suppresses histone lysine demethylases and enhances ID1 expression in cancer stem cells. :: Cell Death Discovery. 2018 ; 巻 4, 番号 1.
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