Polydispersity as a parameter for indicating the thermal stability of proteins by dynamic light scattering

Kohei Shiba, Takuro Niidome, Etsuko Katoh, Hongyu Xiang, Lu Han, Takeshi Mori, Yoshiki Katayama

研究成果: ジャーナルへの寄稿記事

26 引用 (Scopus)

抄録

A physical parameter for predicting the thermal stability of proteins was provided by a new approach using dynamic light scattering (DLS). The relationship between the melting point measured by differential scanning calorimetry (DSC) and the polydispersity of the hydrodynamic diameter determined by DLS analysis was examined. Calmodulin (CaM) and concanavalin A (ConA) were used as model proteins. The melting point measured by DSC, an indicator for thermal stability, increased and the polydispersity decreased on binding of the proteins to specific ligands, suggesting that the polydispersity could be used an indicator to predict thermal stability. In addition, the increase of thermal stability that resulted from forming a complex could be quantified by polydispersity analysis even when the melting point changed only slightly. 2010

元の言語英語
ページ(範囲)659-663
ページ数5
ジャーナルanalytical sciences
26
発行部数6
DOI
出版物ステータス出版済み - 6 2010

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Polydispersity
Dynamic light scattering
Thermodynamic stability
Melting point
Differential scanning calorimetry
Proteins
Calmodulin
Concanavalin A
Carrier Proteins
Hydrodynamics
Ligands

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

これを引用

Polydispersity as a parameter for indicating the thermal stability of proteins by dynamic light scattering. / Shiba, Kohei; Niidome, Takuro; Katoh, Etsuko; Xiang, Hongyu; Han, Lu; Mori, Takeshi; Katayama, Yoshiki.

:: analytical sciences, 巻 26, 番号 6, 06.2010, p. 659-663.

研究成果: ジャーナルへの寄稿記事

Shiba, Kohei ; Niidome, Takuro ; Katoh, Etsuko ; Xiang, Hongyu ; Han, Lu ; Mori, Takeshi ; Katayama, Yoshiki. / Polydispersity as a parameter for indicating the thermal stability of proteins by dynamic light scattering. :: analytical sciences. 2010 ; 巻 26, 番号 6. pp. 659-663.
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