Polymer brush-stabilized polyplex for a siRNA carrier with long blood circulatory half-life

Arihiro Kano, Takeshi Yamano, Won Choi Sun, Atsushi Maruyama

研究成果: Chapter in Book/Report/Conference proceedingConference contribution

3 被引用数 (Scopus)

抄録

Small interfering RNA (siRNA) holds potential as a therapeutic approach to silence targeted gene of disease, but siRNA has limited stability in vivo. Therefore, delivery system of siRNA is the key to siRNA therapeutic application. We attempted to develop a delivery system, which enables siRNA to demonstrate high stability and long blood circulation. We synthesized a series of bottlebrush-type copolymers (BBCs) possessing polycationic backbone (less than 30 wt%) and abundant water-soluble side chains (more than 70 wt%) as siRNA carrier. A siRNA complexed with the BBC was resistant to nuclease and stable in plasma. Especially, the BBC (10 wt% PLL and 90 wt% PEG) having higher grafting ratio (≈ 90 wt%) of water-soluble side chains showed 100-times enhanced stability of siRNA in mouse bloodstream in vivo. Surprisingly, even when the BBC and siRNA separately injected into mouse at 20 min interval, the BBC increased blood half life of the siRNA. These results suggest that the BBC has higher selectivity in its ionic interaction to siRNA than other anionic substance in blood components. To our knowledge, this is the first report of siRNA delivery carrier which prolonged blood circulation of siRNA without resource-consuming preparation process.

本文言語英語
ホスト出版物のタイトルMulti-functional Materials and Structures - International Conference on Multifunctional Materials and Structures
出版社Trans Tech Publications Ltd
ページ762-764
ページ数3
ISBN(印刷版)0878493786, 9780878493784
DOI
出版ステータス出版済み - 2008
イベントMulti-functional Materials and Structures - International Conference on Multifunctional Materials and Structures - Hong Kong, P.R., 中国
継続期間: 7 28 20087 31 2008

出版物シリーズ

名前Advanced Materials Research
47-50 PART 1
ISSN(印刷版)1022-6680

その他

その他Multi-functional Materials and Structures - International Conference on Multifunctional Materials and Structures
Country中国
CityHong Kong, P.R.
Period7/28/087/31/08

All Science Journal Classification (ASJC) codes

  • Engineering(all)

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