Polysaccharide–polynucleotide complexes

Kazuya Koumoto, Taro Kimura, Masami Mizu, Toyoki Kunitake, Kazuo Sakurai, Seiji Shinkai

研究成果: ジャーナルへの寄稿学術誌査読

14 被引用数 (Scopus)

抄録

Recently, we found that schizophyllan (SPG), which is a natural and neutral polysaccharide, can form macromolecular complexes with certain polynucleotides. In order to increase the stability and the affinity of the complexes, we synthesized a 2-aminoethanol-appended SPG (N-s-SPG) by utilizing periodate oxidation and reductive amination. Although the modification level was only 2.4 ± 0.3%, the melting temperature of the poly(C)–N-s-SPG complex was increased by 8 °C comparing with the poly(C)–s-SPG complex. In addition, the conformation of the complex was scarcely perturbed by the introduction of amino groups. When we formed complexes of N-s-SPG and other polynucleotides, the melting temperature of their complexes increased without exception. Moreover, the amino modification induced complexation with poly(U), which does not form a complex with SPG. The affinity for the short chain nucleotide was also enhanced. These results indicate that the present modification method is quite useful to improve complex stability and we believe, therefore, that this strategy will make it possible to apply SPG to a novel gene carrier.

本文言語英語
ページ(範囲)2477-2484
ページ数8
ジャーナルJournal of the Chemical Society. Perkin Transactions 1
2
22
DOI
出版ステータス出版済み - 8月 1 2002

!!!All Science Journal Classification (ASJC) codes

  • 化学 (全般)

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