Polysaccharide/polynucleotide complexes Part 6: Complementary-strand- induced release of single-stranded DNA bound in the schizophyllan complex

Kazuya Koumoto, Masami Mizu, Kazuo Sakurai, Toyoki Kunitake, Seiji Shinkai

研究成果: Contribution to journalArticle査読

14 被引用数 (Scopus)

抄録

Spectroscopic properties of single-stranded DNA/schizophyllan ternary complexes (ss-DNA · 2s-SPG), induced by addition of either complementary or noncomplementary strands, have been investigated. The addition of the complementary strands to ss-DNA · 2s-SPG induced the quickreleas e of the bound ss-DNA to the complementary strands (both DNA and RNA), whereas the ternary complex was unaffected upon addition of noncomplementary strands. Our experiments imply that SPG has complexation properties indispensable to the gene carriers. As far as we know, there is no report on exploitation of such nonviral gene carriers that can accomplish an intelligent release of the bound ss-DNA toward the complementary strands. We believe, therefore, that SPG, a natural and neutral polysaccharide, has a great potential to become a new ss-DNA carrier.

本文言語英語
ページ(範囲)520-529
ページ数10
ジャーナルChemistry and Biodiversity
1
3
DOI
出版ステータス出版済み - 1 1 2004

All Science Journal Classification (ASJC) codes

  • バイオエンジニアリング
  • 生化学
  • 化学 (全般)
  • 分子医療
  • 分子生物学

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