TY - JOUR
T1 - Polysaccharide/polynucleotide complexes Part 6
T2 - Complementary-strand- induced release of single-stranded DNA bound in the schizophyllan complex
AU - Koumoto, Kazuya
AU - Mizu, Masami
AU - Sakurai, Kazuo
AU - Kunitake, Toyoki
AU - Shinkai, Seiji
PY - 2004/1/1
Y1 - 2004/1/1
N2 - Spectroscopic properties of single-stranded DNA/schizophyllan ternary complexes (ss-DNA · 2s-SPG), induced by addition of either complementary or noncomplementary strands, have been investigated. The addition of the complementary strands to ss-DNA · 2s-SPG induced the quickreleas e of the bound ss-DNA to the complementary strands (both DNA and RNA), whereas the ternary complex was unaffected upon addition of noncomplementary strands. Our experiments imply that SPG has complexation properties indispensable to the gene carriers. As far as we know, there is no report on exploitation of such nonviral gene carriers that can accomplish an intelligent release of the bound ss-DNA toward the complementary strands. We believe, therefore, that SPG, a natural and neutral polysaccharide, has a great potential to become a new ss-DNA carrier.
AB - Spectroscopic properties of single-stranded DNA/schizophyllan ternary complexes (ss-DNA · 2s-SPG), induced by addition of either complementary or noncomplementary strands, have been investigated. The addition of the complementary strands to ss-DNA · 2s-SPG induced the quickreleas e of the bound ss-DNA to the complementary strands (both DNA and RNA), whereas the ternary complex was unaffected upon addition of noncomplementary strands. Our experiments imply that SPG has complexation properties indispensable to the gene carriers. As far as we know, there is no report on exploitation of such nonviral gene carriers that can accomplish an intelligent release of the bound ss-DNA toward the complementary strands. We believe, therefore, that SPG, a natural and neutral polysaccharide, has a great potential to become a new ss-DNA carrier.
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U2 - 10.1002/cbdv.200490045
DO - 10.1002/cbdv.200490045
M3 - Article
C2 - 17191866
AN - SCOPUS:9144234149
VL - 1
SP - 520
EP - 529
JO - Chemistry and Biodiversity
JF - Chemistry and Biodiversity
SN - 1612-1872
IS - 3
ER -