Possible involvement of P2Y2 metabotropic receptors in ATP-induced transient receptor potential vanilloid receptor 1-mediated thermal hypersensitivity

Tomoko Moriyama, Tohko Iida, Kimiko Kobayashi, Tomohiro Higashi, Tetsuo Fukuoka, Hideki Tsumura, Catherine Leon, Noboru Suzuki, Kazuhide Inoue, Christian Gachet, Koichi Noguchi, Makoto Tominaga

研究成果: Contribution to journalArticle査読

190 被引用数 (Scopus)

抄録

The capsaicin receptor transient receptor potential V1 (TRPV1; also known as vanilloid receptor 1) is a sensory neuron-specific ion channel that serves as a polymodal detector of pain-producing chemical and physical stimuli. It has been reported that extracellular ATP potentiates the TRPV1 currents evoked by capsaicin or protons and reduces the temperature threshold for its activation through metabotropic P2Y receptors in a PKC-dependent pathway, suggesting that TRPV1 activation could trigger the sensation of pain at normal body temperature in the presence of ATP. Here, we show that ATP-induced thermal hyperalgesia was abolished in mice lacking TRPV1, suggesting the functional interaction between ATP and TRPV1 at a behavioral level. However, thermal hyperalgesia was preserved in P2Y1 receptor-deficient mice. Patch-clamp analyses using mouse dorsal root ganglion neurons indicated the involvement of P2Y2 rather than P2Y1 receptors. Coexpression of TRPV1 mRNA with P2Y2 mRNA, but not P2Y1 mRNA, was determined in the rat lumbar DRG using in situ hybridization histochemistry. These data indicate the importance of metabotropic P2Y2 receptors in nociception through TRPV1.

本文言語英語
ページ(範囲)6058-6062
ページ数5
ジャーナルJournal of Neuroscience
23
14
DOI
出版ステータス出版済み - 7 9 2003

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

フィンガープリント 「Possible involvement of P2Y<sub>2</sub> metabotropic receptors in ATP-induced transient receptor potential vanilloid receptor 1-mediated thermal hypersensitivity」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル