Post-bisulfite Adaptor Tagging Based on an ssDNA Ligation Technique (tPBAT)

Fumihito Miura, Yukiko Shibata, Miki Miura, Takashi Ito

研究成果: 書籍/レポート タイプへの寄稿

抄録

Post-bisulfite adaptor tagging (PBAT) is a concept that enables the preparation of an efficient sequencing library from bisulfite-treated DNA, and it also means the protocol implemented the concept. Although the previous PBAT or rPBAT was sensitive enough for single-cell methylome analysis, the protocol had several drawbacks owing to the repeated random priming reactions. To resolve these problems, we developed a unique single-strand DNA ligation technique, termed TACS ligation, and established a new protocol called tPBAT. With tPBAT, the data quality improved, with a longer insert and higher mapping rate than that obtained with rPBAT. In addition, paired-end sequencing and indexing were supported by the default. In this chapter, the tPBAT protocol is introduced, and a thorough description of its application to small samples is provided.

本文言語英語
ホスト出版物のタイトルMethods in Molecular Biology
出版社Humana Press Inc.
ページ21-37
ページ数17
DOI
出版ステータス出版済み - 2023

出版物シリーズ

名前Methods in Molecular Biology
2577
ISSN(印刷版)1064-3745
ISSN(電子版)1940-6029

!!!All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 遺伝学

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