Post-bisulfite adaptor tagging for PCR-free whole-genome bisulfite sequencing

研究成果: 著書/レポートタイプへの貢献

抄録

Post-bisulfite adaptor tagging (PBAT) is a highly efficient procedure to construct libraries for whole-genome bisulfite sequencing (WGBS). PBAT attaches adaptors to bisulfite-converted genomic DNA to circumvent bisulfite-induced degradation of library DNA inherent to conventional WGBS protocols. Consequently, it enables PCR-free WGBS from nanogram quantities of mammalian DNA, thereby serving as an invaluable tool for methylomics.

元の言語英語
ホスト出版物のタイトルMethods in Molecular Biology
出版者Humana Press Inc.
ページ123-136
ページ数14
DOI
出版物ステータス出版済み - 1 1 2018

出版物シリーズ

名前Methods in Molecular Biology
1708
ISSN(印刷物)1064-3745

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Genome
Polymerase Chain Reaction
Genomic Library
DNA
hydrogen sulfite
Gene Library

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

これを引用

Miura, F., & Ito, T. (2018). Post-bisulfite adaptor tagging for PCR-free whole-genome bisulfite sequencing. : Methods in Molecular Biology (pp. 123-136). (Methods in Molecular Biology; 巻数 1708). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7481-8_7

Post-bisulfite adaptor tagging for PCR-free whole-genome bisulfite sequencing. / Miura, Fumihito; Ito, Takashi.

Methods in Molecular Biology. Humana Press Inc., 2018. p. 123-136 (Methods in Molecular Biology; 巻 1708).

研究成果: 著書/レポートタイプへの貢献

Miura, F & Ito, T 2018, Post-bisulfite adaptor tagging for PCR-free whole-genome bisulfite sequencing. : Methods in Molecular Biology. Methods in Molecular Biology, 巻. 1708, Humana Press Inc., pp. 123-136. https://doi.org/10.1007/978-1-4939-7481-8_7
Miura F, Ito T. Post-bisulfite adaptor tagging for PCR-free whole-genome bisulfite sequencing. : Methods in Molecular Biology. Humana Press Inc. 2018. p. 123-136. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7481-8_7
Miura, Fumihito ; Ito, Takashi. / Post-bisulfite adaptor tagging for PCR-free whole-genome bisulfite sequencing. Methods in Molecular Biology. Humana Press Inc., 2018. pp. 123-136 (Methods in Molecular Biology).
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