@inbook{dee536ae9691416baae07436ff2087fb,
title = "Post-bisulfite Adaptor Tagging with a Highly Efficient Single-Stranded DNA Ligation Technique",
abstract = "Post-bisulfite adaptor tagging (PBAT) is a procedure for efficiently preparing a sequencing library for whole-genome bisulfite sequencing (WGBS). The original version of the PBAT protocol was highly efficient, such that it helped realize library preparation from samples of limited amounts. However, two rounds of random priming reactions employed in the original protocol limited further improvement of the PBAT protocol in terms of read length and mapping rate. In this chapter, an improved version of the PBAT protocol called tPBAT is described.",
author = "Fumihito Miura and Takashi Ito",
note = "Funding Information: We are grateful for Yukiko Shibata and Miki Miura for their technical supports. This work was supported by a JSPS KAKENHI grant to F.M. (20H03243) and T.I. (17H06305). This work was also supported by the Platform Project for Supporting Drug Discovery and Life Science Research (Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS)) from AMED under Grant Number JP20am0101103. Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-2815-7_4",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "45--57",
booktitle = "Methods in Molecular Biology",
}