Post-bisulfite Adaptor Tagging with a Highly Efficient Single-Stranded DNA Ligation Technique

研究成果: 書籍/レポート タイプへの寄稿

抄録

Post-bisulfite adaptor tagging (PBAT) is a procedure for efficiently preparing a sequencing library for whole-genome bisulfite sequencing (WGBS). The original version of the PBAT protocol was highly efficient, such that it helped realize library preparation from samples of limited amounts. However, two rounds of random priming reactions employed in the original protocol limited further improvement of the PBAT protocol in terms of read length and mapping rate. In this chapter, an improved version of the PBAT protocol called tPBAT is described.

本文言語英語
ホスト出版物のタイトルMethods in Molecular Biology
出版社Humana Press Inc.
ページ45-57
ページ数13
DOI
出版ステータス出版済み - 2023

出版物シリーズ

名前Methods in Molecular Biology
2594
ISSN(印刷版)1064-3745
ISSN(電子版)1940-6029

!!!All Science Journal Classification (ASJC) codes

  • 分子生物学
  • 遺伝学

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