Post-translational modifications of the N-terminal tail of histone H3 in holocentric chromosomes of Bombyx mori

Hiroaki Mon, Makiko Izumi, Hitoshi Mitsunobu, Tsuneyuki Tatsuke, Kazuhiro Iiyama, Hiroyuki Jikuya, Jae Man Lee, Takahiro Kusakabe

研究成果: Contribution to journalArticle査読

2 被引用数 (Scopus)

抄録

Epigenetic information is encoded in post-translational modifications (PTMs) of histones. Various combinations of these marks contribute to the regulation of chromatin-templated DNA metabolisms. The histone code is gradually translated into biological responses in model organisms. However, in the silkworm, the modifications of histones with unique holocentric chromosomes have not yet been analyzed. TAU-PAGE analysis of the silkworm histone variants H2A, H2B, and H3, separated by RP-HPLC, suggested silkworm specific modification. Detailed mass spectrometry analyses of the peptides derived from the N-terminus of the silkworm H3.2 generated by glutamyl endopeptidase, lysyl endopeptidase, and trypsin digestions revealed global modifications around H3K9.

本文言語英語
ページ(範囲)902-908
ページ数7
ジャーナルInsect Biochemistry and Molecular Biology
41
11
DOI
出版ステータス出版済み - 11 2011

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学
  • 昆虫科学

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