Postischemic gene transfer of midkine, a neurotrophic factor, protects against focal brain ischemia

J. Takada, H. Ooboshi, T. Ago, T. Kitazono, H. Yao, K. Kadomatsu, T. Muramatsu, S. Ibayashi, M. Iida

研究成果: Contribution to journalArticle査読

37 被引用数 (Scopus)


Gene therapy may be a promising approach for treatment of brain ischemia. In this study, we examined the effect of postischemic gene transfer of midkine, a heparin-binding neurotrophic factor, using a focal brain ischemia model with the photothrombotic occlusion method. At 90 min after induction of brain ischemia in spontaneously hypertensive rats, a replication-deficient recombinant adenovirus encoding mouse midkine (AdMK, n = 7) or a control vector encoding β-galactosidase (Adβgal, n = 7) was injected into the lateral ventricle ipsilateral to ischemia. At 2 days after ischemia, we determined infarct volume by 2,3,5-triphenyltetrazolium chloride staining. There were no significant differences in cerebral blood flow 1 h after ischemia between AdMK and Adβgal groups. Infarct volume of AdMK group was 51±27 mm3, which was significantly smaller than that of Adβgal group (86±27 mm3, P < 0.05). TUNEL-positive and cleaved caspase-3-positive cells in the periischemic area of AdMK-treated rats were significantly fewer than those in Adβgal-treated rats, suggesting that the reduction of infarct volume by midkine was partly mediated by its antiapoptotic action. Thus, gene transfer of midkine to the ischemic brain may be effective in the treatment of brain ischemia.

ジャーナルGene Therapy
出版ステータス出版済み - 3 2005

All Science Journal Classification (ASJC) codes

  • 分子医療
  • 分子生物学
  • 遺伝学


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