Potent cytotoxic effect of the trans10, cis12 isomer of conjugated linoleic acid on rat hepatoma dRLh-84 cells

Masao Yamasaki, Hitomi Chujo, Yasuko Koga, Ayana Oishi, Tatsuya Rikimaru, Mitsuo Shimada, Keizo Sugimachi, Hirofumi Tachibana, Koji Yamada

研究成果: ジャーナルへの寄稿記事

46 引用 (Scopus)

抜粋

We evaluated the cytotoxic effect of conjugated linoleic acid (CLA) on rat hepatoma dRLh-84 cells in vitro. When cells were cultured in the presence of CLA, strong cytotoxic effect on dRLh-84 cells was recognized at 1μM level compared to the control vehicle group, and trans10, cis12-CLA but not cis9, trans11-CLA was shown to be an active isomer for inducing this effect. Increase of the sub-G1 population and activation of caspase-3 and 9 accompanied with a time-dependent cleavage of poly(ADP-ribose) polymerase were recognized in dRLh-84 cells treated with trans10, cis12-CLA. In addition, we could see nuclear fragmentation in dRLh-84 cells treated with trans10, cis12-CLA by laser scanning confocal microscopy observation. Cytotoxic effect of trans10, cis12-CLA on normal hepatocytes was weaker than on dRLh-84 cells. These data indicate trans10, cis12-CLA has a potent cytotoxic effect on dRLh-84 cells through at least in part by an apoptotic pathway.

元の言語英語
ページ(範囲)171-180
ページ数10
ジャーナルCancer Letters
188
発行部数1-2
DOI
出版物ステータス出版済み - 12 15 2002

    フィンガープリント

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

これを引用

Yamasaki, M., Chujo, H., Koga, Y., Oishi, A., Rikimaru, T., Shimada, M., ... Yamada, K. (2002). Potent cytotoxic effect of the trans10, cis12 isomer of conjugated linoleic acid on rat hepatoma dRLh-84 cells. Cancer Letters, 188(1-2), 171-180. https://doi.org/10.1016/S0304-3835(02)00459-7