Practical application of fluorescence-based image analyzer for PCR single-stranded conformation polymorphism analysis used in detection of multiple point mutations

Asako Takahashi-Fujii, Yoshizumi Ishino, Atsushi Shimada, Ikunoshin Kato

研究成果: ジャーナルへの寄稿記事

14 引用 (Scopus)

抄録

We present a practical application of polymerase chain reaction single-strand conformational polymorphism (PCR-SSCP) using a fluorescence-based image analyzer. Fluorescence-labeled primers were used to amplify target sequences. After nondenaturing polyacrylamide gel electrophoresis using a conventional sequencing gel apparatus, the glass plate was directly transferred from the gel box to the image analyzer. High-quality computer storage of the imaging data allowed the image intensity to be quantified over a wide range. Various data bases can be constructed on the basis of this imaging data. This system is suitable for use in clinical examination settings, and its application, to detect ras gene mutations in human pancreatic cancer specimens, is presented.

元の言語英語
ページ(範囲)323-327
ページ数5
ジャーナルGenome Research
2
発行部数4
DOI
出版物ステータス出版済み - 1 1 1993

Fingerprint

Point Mutation
Fluorescence
Gels
Polymerase Chain Reaction
ras Genes
Information Storage and Retrieval
Pancreatic Neoplasms
Glass
Polyacrylamide Gel Electrophoresis
Databases
Mutation

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

これを引用

Practical application of fluorescence-based image analyzer for PCR single-stranded conformation polymorphism analysis used in detection of multiple point mutations. / Takahashi-Fujii, Asako; Ishino, Yoshizumi; Shimada, Atsushi; Kato, Ikunoshin.

:: Genome Research, 巻 2, 番号 4, 01.01.1993, p. 323-327.

研究成果: ジャーナルへの寄稿記事

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