Precise 1H- and 13C-NMR reassignment of dehydrocrebanine by 10-mg INADEQUATE and in silico analysis: With an alert for its toxicity

Chotika Phupong, Masahiko Suenaga, Phuangthip Bhoopong, Warangkana Chunglok, Gunlanan Jaritngam, Milandip Karak, Keiichi Yoshida, Worrapong Phupong, Kohei Torikai

研究成果: Contribution to journalArticle

抄録

During our continuous investigation on a Thai traditional medicinal plant, Stephania venosa Spreng, an alkaloid that seemed to be O-methyldehydrobulbocapnine (MDB) or dehydrocrebanine (DC), had been isolated. However, none of the previously reported 1H- and 13C-NMR data for MDB and DC matched our charts. Thus, we herein report the unambiguous determination of the alkaloid structure as DC via a successful INADEQUATE experiment using 10 mg of the unenriched natural specimen as well as an in silico estimation. Furthermore, our biological assay revealed that both the dichloromethane extract of S. venosa and the isolated DC show cytotoxicity to murine macrophage cells, which should be alerted to a folk doctor community that are using S. venosa to treat patients.

本文言語英語
論文番号131310
ジャーナルTetrahedron
76
27
DOI
出版ステータス出版済み - 7 3 2020

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Drug Discovery
  • Organic Chemistry

フィンガープリント 「Precise <sup>1</sup>H- and <sup>13</sup>C-NMR reassignment of dehydrocrebanine by 10-mg INADEQUATE and in silico analysis: With an alert for its toxicity」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

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