Preliminary evaluation of an improved enzymatic assay method for measuring potassium concentrations in serum

Eri Ota, Shin ichi Sakasegawa, Shigeru Ueda, Kenji Konishi, Masaru Akimoto, Takiko Tateishi, Miki Kawano, Eisaku Hokazono, Yuzo Kayamori

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

Background: K+ has important physiological functions. K+ concentrations in serum are generally determined using ion-selective electrodes (ISEs), though measurement using reagents in aqueous medium is also useful. Methods: K+ concentrations were measured using recombinant inosine 5'-monophosphate dehydrogenase (IMPDH), which was activated only by K+ and NH4+. Exogenous NH4+ and endogenous NH4+ were eliminated using glutamine synthase. Results: Regression analysis of the enzymatic assay (y) vs. the ISE method (x) gave the following relation: y=1.03x+0.09 (n=54, Sy,x=0.06mmol/l). The linear range was up to 12mmol/l when 1U/ml IMPDH was used. Conclusion: Advantages of the proposed assay method are: (i) the measured range is wider than that of existing enzymatic methods; (ii) the conditions for K+ determination can be maintained constant, regardless of the amount of NH4+ in the analyte and reagents; and (iii) the elimination system is simpler because the recombinant IMPDH is stimulated by only K+ and NH4+ and is unaffected by biological materials.

元の言語英語
ページ(範囲)73-75
ページ数3
ジャーナルClinica Chimica Acta
446
DOI
出版物ステータス出版済み - 6 5 2015

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IMP Dehydrogenase
Enzyme Assays
Assays
Potassium
Ion-Selective Electrodes
Serum
Glutamine
Regression analysis
Biological materials
Regression Analysis

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

これを引用

Ota, E., Sakasegawa, S. I., Ueda, S., Konishi, K., Akimoto, M., Tateishi, T., ... Kayamori, Y. (2015). Preliminary evaluation of an improved enzymatic assay method for measuring potassium concentrations in serum. Clinica Chimica Acta, 446, 73-75. https://doi.org/10.1016/j.cca.2015.03.042

Preliminary evaluation of an improved enzymatic assay method for measuring potassium concentrations in serum. / Ota, Eri; Sakasegawa, Shin ichi; Ueda, Shigeru; Konishi, Kenji; Akimoto, Masaru; Tateishi, Takiko; Kawano, Miki; Hokazono, Eisaku; Kayamori, Yuzo.

:: Clinica Chimica Acta, 巻 446, 05.06.2015, p. 73-75.

研究成果: ジャーナルへの寄稿記事

Ota, E, Sakasegawa, SI, Ueda, S, Konishi, K, Akimoto, M, Tateishi, T, Kawano, M, Hokazono, E & Kayamori, Y 2015, 'Preliminary evaluation of an improved enzymatic assay method for measuring potassium concentrations in serum', Clinica Chimica Acta, 巻. 446, pp. 73-75. https://doi.org/10.1016/j.cca.2015.03.042
Ota, Eri ; Sakasegawa, Shin ichi ; Ueda, Shigeru ; Konishi, Kenji ; Akimoto, Masaru ; Tateishi, Takiko ; Kawano, Miki ; Hokazono, Eisaku ; Kayamori, Yuzo. / Preliminary evaluation of an improved enzymatic assay method for measuring potassium concentrations in serum. :: Clinica Chimica Acta. 2015 ; 巻 446. pp. 73-75.
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abstract = "Background: K+ has important physiological functions. K+ concentrations in serum are generally determined using ion-selective electrodes (ISEs), though measurement using reagents in aqueous medium is also useful. Methods: K+ concentrations were measured using recombinant inosine 5'-monophosphate dehydrogenase (IMPDH), which was activated only by K+ and NH4+. Exogenous NH4+ and endogenous NH4+ were eliminated using glutamine synthase. Results: Regression analysis of the enzymatic assay (y) vs. the ISE method (x) gave the following relation: y=1.03x+0.09 (n=54, Sy,x=0.06mmol/l). The linear range was up to 12mmol/l when 1U/ml IMPDH was used. Conclusion: Advantages of the proposed assay method are: (i) the measured range is wider than that of existing enzymatic methods; (ii) the conditions for K+ determination can be maintained constant, regardless of the amount of NH4+ in the analyte and reagents; and (iii) the elimination system is simpler because the recombinant IMPDH is stimulated by only K+ and NH4+ and is unaffected by biological materials.",
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AU - Sakasegawa, Shin ichi

AU - Ueda, Shigeru

AU - Konishi, Kenji

AU - Akimoto, Masaru

AU - Tateishi, Takiko

AU - Kawano, Miki

AU - Hokazono, Eisaku

AU - Kayamori, Yuzo

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AB - Background: K+ has important physiological functions. K+ concentrations in serum are generally determined using ion-selective electrodes (ISEs), though measurement using reagents in aqueous medium is also useful. Methods: K+ concentrations were measured using recombinant inosine 5'-monophosphate dehydrogenase (IMPDH), which was activated only by K+ and NH4+. Exogenous NH4+ and endogenous NH4+ were eliminated using glutamine synthase. Results: Regression analysis of the enzymatic assay (y) vs. the ISE method (x) gave the following relation: y=1.03x+0.09 (n=54, Sy,x=0.06mmol/l). The linear range was up to 12mmol/l when 1U/ml IMPDH was used. Conclusion: Advantages of the proposed assay method are: (i) the measured range is wider than that of existing enzymatic methods; (ii) the conditions for K+ determination can be maintained constant, regardless of the amount of NH4+ in the analyte and reagents; and (iii) the elimination system is simpler because the recombinant IMPDH is stimulated by only K+ and NH4+ and is unaffected by biological materials.

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