Preliminary study on in situ realtime quantitation of target bacteria on the principle of flow cytometry

Gen Murakami, Yuichi Sugai, Kyuro Sasaki

研究成果: Chapter in Book/Report/Conference proceedingConference contribution


In-situ realtime method that can monitor the target bacteria should be used to determine the real situation of the bacteria in deep parts of heaps in heap bioleaching plants. This study suggest to apply flow cytometry technology to in-situ realtime monitoring of target bacteria. Flow cytometry is a method that can rapidly quantify the bacterial cells in bacterial suspension based on the detection of lights that are emitted from bacterial cells. In this study, we estimated the possibility of the application of flow cytometry to the selective detection of target bacteria. The bacterial culture solution that had been diluted by water including other bacteria was provided for fluorescence spectral analysis and scattered light analysis that were functions of flow cytometry. Our target bacteria could be selectively detected by those analyses in this study, therefore, it was shown that the flow cytometry could be useful for detecting target bacteria selectively. Because the measurement principle of flow cytometry is quite simple, it can be expected to be installed into deep heaps through the monitoring wells and determine the dominance of target bacteria in-situ and realtime in the future.

ホスト出版物のタイトル22nd International Biohydrometallurgy Symposium
編集者Sabrina Hedrich, Axel Schippers, Kathrin Rubberdt, Franz Glombitza, Wolfgang Sand, Wolfgang Sand, Mario Vera Veliz, Sabine Willscher
Place of Publication Switzerland
出版社Trans Tech Publications Ltd
出版ステータス出版済み - 8 2017
イベント22nd International Biohydrometallurgy Symposium, 2017 - Freiberg, ドイツ
継続期間: 9 24 20179 27 2017


名前Solid State Phenomena
262 SSP


その他22nd International Biohydrometallurgy Symposium, 2017

All Science Journal Classification (ASJC) codes

  • Fuel Technology
  • Biochemistry

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