Prenatal administration of neuropeptide bombesin promotes lung development in a rat model of nitrofen-induced congenital diaphragmatic hernia

Kohei Sakai, Osamu Kimura, Taizo Furukawa, Shigehisa Fumino, Koji Higuchi, Junko Wakao, Koseki Kimura, Shigeyoshi Aoi, Kouji Masumoto, Tatsuro Tajiri

研究成果: ジャーナルへの寄稿学術誌査読

7 被引用数 (Scopus)

抄録

Background/purpose Fetal medical treatment to improve lung hypoplasia in congenital diaphragmatic hernia (CDH) has yet to be established. The neuropeptide bombesin (BBS) might play an important role in lung development. The present study aims to determine whether prenatally administered BBS could be useful to promote fetal lung development in a rat model of nitrofen-induced CDH. Methods Pregnant rats were administered with nitrofen (100 mg) on gestation day 9.5 (E9.5). BBS (50 mg/kg/day) was then daily infused intraperitoneally from E14, and fetal lungs were harvested on E21. The expression of PCNA was assessed by both immunohistochemical staining and RT-PCR to determine the amount of cell proliferation. Lung maturity was assessed as the expression of TTF-1, a marker of alveolar epithelial cell type II. Results The lung-body-weight ratio was significantly increased in CDH/BBS(+) compared with CDH/BBS(-) (p < 0.05). The number of cells stained positive for PCNA and TTF-1 was significantly decreased in CDH/BBS(+) compared with CDH/BBS(-) (p < 0.01). The TTF-1 mRNA expression levels were significantly decreased in CDH/BBS(+) compared with CDH/BBS(-) (p < 0.05). Conclusions Prenatally administered BBS promotes lung development in a rat model of nitrofen-induced CDH. Neuropeptide BBS could help to rescue lung hypoplasia in fetal CDH.

本文言語英語
ページ(範囲)1749-1752
ページ数4
ジャーナルJournal of Pediatric Surgery
49
12
DOI
出版ステータス出版済み - 12月 1 2014
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 外科
  • 小児科学、周産期医学および子どもの健康

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