Preparation and cell culture behavior of self-assembled monolayers composed of chitohexaose and chitosan hexamer

Yuka Yoshiike, Shingo Yokota, Nobuo Tanaka, Takuya Kitaoka, Hiroyuki Wariishi

研究成果: Contribution to journalArticle

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Chitohexaose and chitosan hexamer were site-selectively modified at each reducing end with thiosemicarbazide, followed by self-assembly chemisorption of the S-derivatives on a gold substrate via S-Au bonding. Quartz crystal microbalance analysis and X-ray photoelectron spectroscopy suggested successful formation of self-assembled monolayers (SAMs) consisting of chitin and chitosan with sugar densities 0.39 and 0.35 chains nm-2, respectively. As-prepared SAMs were very hydrophilic (with water contact angles 12-13°) and flat at the nanometer scale (RMS ≈ 1.7 nm). Mouse fibroblasts (NIH-3T3) preferentially adhered to both SAMs, while fewer cells were attached to the more hydrophobic, intact gold substrate. The fixed carbohydrates induced good proliferation of NIH-3T3 cells, while surface treatment with free sugars had almost no positive effects. This architectural design of water-soluble oligosaccharide-SAMs is expected to provide a new approach for functional development of carbohydrate-decorated biointerfaces.

元の言語英語
ページ(範囲)21-27
ページ数7
ジャーナルCarbohydrate Polymers
82
発行部数1
DOI
出版物ステータス出版済み - 8 2 2010

All Science Journal Classification (ASJC) codes

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

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